Decline of follicular oocyte maturation inhibitor coincident with maturation and achievement of fertilizability of oocytes recovered at midcycle of gonadotropin-treated women

C. P. Channing, C. Q. Liu, G. S. Jones, H. Jones

Research output: Contribution to journalArticlepeer-review

Abstract

To examine whether a decline in follicular oocyte maturation inhibitor (OMI) is associated with attainment of oocyte maturation and fertilizability, OMI was measured in follicular fluid (FF) of 39 follicles of 20 normal women given human menopausal gonadotrophin and human chorionic gonadotropin to induce follicular growth and maturation. Oocytes were aspirated per laparoscope, the fluid was saved, and the egg was observed, incubated, and inseminated with the husband's sperm. Concepti that developed to the 4- to 8-cell stage were transferred to the uterus and the women were followed for pregnancy. OMI activity in each FF was measured by using cultured cumulus-enclosed porcine oocytes (30-40 oocytes per FF sample). Estrogen, progesterone, and δ4-androstenedione were measured in FF by radioimmunoassay. The FF of 13 preovulatory follicles yielding oocytes that were mature and fertilizable had significantly less OMI activity (mean ± SEM) (0.58 ± 0.10 unit/ml) compared to follicles yielding immature oocytes (2.8 ± 0.56 units/ml; n =9), atretic oocytes (5.5 ± 2.5 units/ml; n = 7), or preovulatory oocytes with fractured zonae (1.9 ± 0.63 units/ml; n = 7). The estrogen concentration (mean ± SEM) of prevulatory follicles yielding mature fertilizable eggs or mature eggs with fractured zonae was greater (396 ± 34 ng/ml; n = 20) compared to follicles yielding immature or atretic eggs (203 ± 59 ng/ml; n = 9 and 97 ± 47 ng/ml; n = 7, respectively; P<0.05). Progesterone concentration (mean ± SEM; ng/ml) of FF was generally elevated in all preovulatory follicles (635 ± 53) compared to immature or atretic follicles (230 ± 64 and 76 ± 17, respectively; P<0.05). It may be concluded that in normal follicle maturation there is a decline in OMI in the follicle containing an oocyte that becomes mature and fertilizable. There is also an increase in estrogen, progesterone, and follicle size. It is also possible to have an abnormal follicle maturation when there is an increase in size as well as FF, estrogen, and progesterone, but without a decline in OMI-a situation which can lead to production of a nonfertilizable oocyte.

Original languageEnglish (US)
Pages (from-to)4184-4188
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number13 I
DOIs
StatePublished - 1982

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Decline of follicular oocyte maturation inhibitor coincident with maturation and achievement of fertilizability of oocytes recovered at midcycle of gonadotropin-treated women'. Together they form a unique fingerprint.

Cite this