Deciphering the Roles of N-Glycans on Collagen-Platelet Interactions

Christian Toonstra, Yingwei Hu, Hui Zhang

Research output: Contribution to journalArticle

Abstract

Collagen is a potent agonist for platelet activation, presenting itself as a key contributor to coagulation via interactions with platelet glycoproteins. The fine details dictating platelet-collagen interactions are poorly understood. In particular, glycosylation could be a key determinant in the platelet-collagen interaction. Here, we report an affinity purification coupled to a mass spectrometry-based approach to elucidate the function of N-glycans in dictating platelet-collagen interactions. By integrative proteomic and glycoproteomic analysis of collagen-platelet interactive proteins with N-glycan manipulation, we demonstrate that the interaction of platelet adhesive receptors with collagen is highly N-glycan regulated, with glycans on many receptors playing positive roles in collagen binding, with glycans on other platelet glycoproteins exhibiting inhibitory roles on the binding to collagen. Our results significantly enhance our understanding of the details of glycans influencing the platelet-collagen interaction.

Original languageEnglish (US)
Pages (from-to)2467-2477
Number of pages11
JournalJournal of proteome research
Volume18
Issue number6
DOIs
StatePublished - Jun 7 2019

Keywords

  • N-glycans
  • affinity purification
  • collagen
  • collagen-platelet interactions
  • glycoproteins
  • glycoproteomics
  • intact glycopeptides
  • mass spectrometry
  • platelet
  • protein-protein interactions

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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