Decellularization of bovine corneas for tissue engineering applications

Sara Ponce Márquez, Virginia Sáez Martínez, Winnette McIntosh Ambrose, Jennie Wang, Nerea Garagorri Gantxegui, Oliver D Schein, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Scaffolds derived from processed tissues offer viable alternatives to synthetic polymers as biological scaffolds for regenerative medicine. Tissue-derived scaffolds provide an extracellular matrix (ECM) as the starting material for wound healing and the functional reconstruction of tissues, offering a potentially valuable approach for the replacement of damaged or missing tissues. Additionally, acellular tissue may provide a natural microenvironment for host-cell migration and the induction of stem cell differentiation to contribute to tissue regeneration. There are a number of processing methods that aim to stabilize and provide an immunologically inert tissue scaffold. Furthermore, these tissue-processing methods can often be applied to xenogenic transplants because the essential components of the ECM are often maintained between species. In this study, we applied several tissue-processing protocols to the cornea in order to obtain a decellularized cornea matrix that maintained the clarity and mechanical properties of the native tissue. Histology, mechanical testing and electron microscopy techniques were used to assess the cell extraction process and the organization of the remaining ECM. In vitro cell seeding experiments confirmed the processed corneas' biocompatibility.

Original languageEnglish (US)
Pages (from-to)1839-1847
Number of pages9
JournalActa Biomaterialia
Volume5
Issue number6
DOIs
StatePublished - Jul 2009

Fingerprint

Tissue Engineering
Tissue engineering
Cornea
Tissue
Tissue Scaffolds
Extracellular Matrix
Scaffolds
Processing
Tissue regeneration
Transplants
Histology
Regenerative Medicine
Mechanical testing
Scaffolds (biology)
Stem cells
Biocompatibility
Wound Healing
Electron microscopy
Cell Movement
Regeneration

Keywords

  • Biomaterial
  • Collagen
  • Cornea
  • Decellularized
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Ponce Márquez, S., Martínez, V. S., McIntosh Ambrose, W., Wang, J., Gantxegui, N. G., Schein, O. D., & Elisseeff, J. H. (2009). Decellularization of bovine corneas for tissue engineering applications. Acta Biomaterialia, 5(6), 1839-1847. https://doi.org/10.1016/j.actbio.2009.02.011

Decellularization of bovine corneas for tissue engineering applications. / Ponce Márquez, Sara; Martínez, Virginia Sáez; McIntosh Ambrose, Winnette; Wang, Jennie; Gantxegui, Nerea Garagorri; Schein, Oliver D; Elisseeff, Jennifer Hartt.

In: Acta Biomaterialia, Vol. 5, No. 6, 07.2009, p. 1839-1847.

Research output: Contribution to journalArticle

Ponce Márquez, S, Martínez, VS, McIntosh Ambrose, W, Wang, J, Gantxegui, NG, Schein, OD & Elisseeff, JH 2009, 'Decellularization of bovine corneas for tissue engineering applications', Acta Biomaterialia, vol. 5, no. 6, pp. 1839-1847. https://doi.org/10.1016/j.actbio.2009.02.011
Ponce Márquez S, Martínez VS, McIntosh Ambrose W, Wang J, Gantxegui NG, Schein OD et al. Decellularization of bovine corneas for tissue engineering applications. Acta Biomaterialia. 2009 Jul;5(6):1839-1847. https://doi.org/10.1016/j.actbio.2009.02.011
Ponce Márquez, Sara ; Martínez, Virginia Sáez ; McIntosh Ambrose, Winnette ; Wang, Jennie ; Gantxegui, Nerea Garagorri ; Schein, Oliver D ; Elisseeff, Jennifer Hartt. / Decellularization of bovine corneas for tissue engineering applications. In: Acta Biomaterialia. 2009 ; Vol. 5, No. 6. pp. 1839-1847.
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