Abstract
Here we report next-generation based whole genome sequencing of two individuals (H1 and H2) from a family of Pakistani descent. The genomic DNA was used to prepare paired-end libraries for whole-genome sequencing. Deep sequencing yielded 706.49 and 778.12 million mapped reads corresponding to 70.64 and 77.81 Gb sequence data and 23 × and 25 × average coverage for H1 and H2, respectively. Notably, a total of 448,544 and 470,683 novel variants, not present in the single nucleotide polymorphism database (dbSNP), were identified in H1 and H2, respectively. Comparative analysis identified 2,415,852 variants common in both genomes including 240,181 variants absent in the dbSNP. Principal component analysis linked the ancestry of both genomes with South Asian populations. In conclusion, we report whole genome sequences of two individuals from a family of Pakistani descent.
| Original language | English (US) |
|---|---|
| Article number | 180174 |
| Journal | Scientific data |
| Volume | 5 |
| DOIs | |
| State | Published - Sep 11 2018 |
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ASJC Scopus subject areas
- Statistics and Probability
- Information Systems
- Education
- Computer Science Applications
- Statistics, Probability and Uncertainty
- Library and Information Sciences
Cite this
Data descriptor : Whole genome sequencing data for two individuals of Pakistani descent. / Khan, Shahid; Kabir, Firoz; M’Hamdi, Oussama; Jiao, Xiaodong; Naeem, Muhammad Asif; Khan, Shaheen N.; Riazuddin, Sheikh; Hejtmancik, J. Fielding; Riazuddin, Sheikh Amer.
In: Scientific data, Vol. 5, 180174, 11.09.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Data descriptor
T2 - Whole genome sequencing data for two individuals of Pakistani descent
AU - Khan, Shahid
AU - Kabir, Firoz
AU - M’Hamdi, Oussama
AU - Jiao, Xiaodong
AU - Naeem, Muhammad Asif
AU - Khan, Shaheen N.
AU - Riazuddin, Sheikh
AU - Hejtmancik, J. Fielding
AU - Riazuddin, Sheikh Amer
PY - 2018/9/11
Y1 - 2018/9/11
N2 - Here we report next-generation based whole genome sequencing of two individuals (H1 and H2) from a family of Pakistani descent. The genomic DNA was used to prepare paired-end libraries for whole-genome sequencing. Deep sequencing yielded 706.49 and 778.12 million mapped reads corresponding to 70.64 and 77.81 Gb sequence data and 23 × and 25 × average coverage for H1 and H2, respectively. Notably, a total of 448,544 and 470,683 novel variants, not present in the single nucleotide polymorphism database (dbSNP), were identified in H1 and H2, respectively. Comparative analysis identified 2,415,852 variants common in both genomes including 240,181 variants absent in the dbSNP. Principal component analysis linked the ancestry of both genomes with South Asian populations. In conclusion, we report whole genome sequences of two individuals from a family of Pakistani descent.
AB - Here we report next-generation based whole genome sequencing of two individuals (H1 and H2) from a family of Pakistani descent. The genomic DNA was used to prepare paired-end libraries for whole-genome sequencing. Deep sequencing yielded 706.49 and 778.12 million mapped reads corresponding to 70.64 and 77.81 Gb sequence data and 23 × and 25 × average coverage for H1 and H2, respectively. Notably, a total of 448,544 and 470,683 novel variants, not present in the single nucleotide polymorphism database (dbSNP), were identified in H1 and H2, respectively. Comparative analysis identified 2,415,852 variants common in both genomes including 240,181 variants absent in the dbSNP. Principal component analysis linked the ancestry of both genomes with South Asian populations. In conclusion, we report whole genome sequences of two individuals from a family of Pakistani descent.
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UR - http://www.scopus.com/inward/citedby.url?scp=85053309936&partnerID=8YFLogxK
U2 - 10.1038/sdata.2018.174
DO - 10.1038/sdata.2018.174
M3 - Article
C2 - 30204152
AN - SCOPUS:85053309936
VL - 5
JO - Scientific data
JF - Scientific data
SN - 2052-4463
M1 - 180174
ER -