TY - JOUR
T1 - DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity
AU - Van Rossum, Damian B.
AU - Patterson, Randen L.
AU - Cheung, King Ho
AU - Barrow, Roxanne K.
AU - Syrovatkina, Viktoriya
AU - Gessell, Gregory S.
AU - Burkholder, Scott G.
AU - Watkins, D. Neil
AU - Foskett, J. Kevin
AU - Snyder, Solomon H.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed.
AB - We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed.
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U2 - 10.1074/jbc.M608760200
DO - 10.1074/jbc.M608760200
M3 - Article
C2 - 16990268
AN - SCOPUS:33846031944
SN - 0021-9258
VL - 281
SP - 37111
EP - 37116
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 48
ER -