DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity

Damian B. Van Rossum, Randen L. Patterson, King Ho Cheung, Roxanne K Barrow, Viktoriya Syrovatkina, Gregory S. Gessell, Scott G. Burkholder, D. Neil Watkins, J. Kevin Foskett, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed.

Original languageEnglish (US)
Pages (from-to)37111-37116
Number of pages6
JournalJournal of Biological Chemistry
Volume281
Issue number48
DOIs
StatePublished - Dec 1 2006

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Cloning
Cell Lineage
Organism Cloning
Assays
Membrane Proteins
Cell Membrane
Membranes
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry

Cite this

DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity. / Van Rossum, Damian B.; Patterson, Randen L.; Cheung, King Ho; Barrow, Roxanne K; Syrovatkina, Viktoriya; Gessell, Gregory S.; Burkholder, Scott G.; Watkins, D. Neil; Foskett, J. Kevin; Snyder, Solomon H.

In: Journal of Biological Chemistry, Vol. 281, No. 48, 01.12.2006, p. 37111-37116.

Research output: Contribution to journalArticle

Van Rossum, DB, Patterson, RL, Cheung, KH, Barrow, RK, Syrovatkina, V, Gessell, GS, Burkholder, SG, Watkins, DN, Foskett, JK & Snyder, SH 2006, 'DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity', Journal of Biological Chemistry, vol. 281, no. 48, pp. 37111-37116. https://doi.org/10.1074/jbc.M608760200
Van Rossum, Damian B. ; Patterson, Randen L. ; Cheung, King Ho ; Barrow, Roxanne K ; Syrovatkina, Viktoriya ; Gessell, Gregory S. ; Burkholder, Scott G. ; Watkins, D. Neil ; Foskett, J. Kevin ; Snyder, Solomon H. / DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 48. pp. 37111-37116.
@article{cf3cc16c74864880b7985d507202d6b0,
title = "DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity",
abstract = "We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed.",
author = "{Van Rossum}, {Damian B.} and Patterson, {Randen L.} and Cheung, {King Ho} and Barrow, {Roxanne K} and Viktoriya Syrovatkina and Gessell, {Gregory S.} and Burkholder, {Scott G.} and Watkins, {D. Neil} and Foskett, {J. Kevin} and Snyder, {Solomon H}",
year = "2006",
month = "12",
day = "1",
doi = "10.1074/jbc.M608760200",
language = "English (US)",
volume = "281",
pages = "37111--37116",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "48",

}

TY - JOUR

T1 - DANGER, a novel regulatory protein of inositol 1,4,5-trisphosphate-receptor activity

AU - Van Rossum, Damian B.

AU - Patterson, Randen L.

AU - Cheung, King Ho

AU - Barrow, Roxanne K

AU - Syrovatkina, Viktoriya

AU - Gessell, Gregory S.

AU - Burkholder, Scott G.

AU - Watkins, D. Neil

AU - Foskett, J. Kevin

AU - Snyder, Solomon H

PY - 2006/12/1

Y1 - 2006/12/1

N2 - We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed.

AB - We report the cloning and characterization of DANGER, a novel protein which physiologically binds to inositol 1,4,5-trisphosphate receptors (IP 3R). DANGER is a membrane-associated protein predicted to contain a partial MAB-21 domain. It is expressed in a wide variety of neuronal cell lineages where it localizes to membranes in the cell periphery together with IP3R. DANGER interacts with IP3R in vitro and co-immunoprecipitates with IP3R from cellular preparations. DANGER robustly enhances Ca2+-mediated inhibition of IP3R Ca 2+ release without affecting IP3 binding in microsomal assays and inhibits gating in single-channel recordings of IP3R. DANGER appears to allosterically modulate the sensitivity of IP3R to Ca2+ inhibition, which likely alters IP3R-mediated Ca 2+ dynamics in cells where DANGER and IP3R are co-expressed.

UR - http://www.scopus.com/inward/record.url?scp=33846031944&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846031944&partnerID=8YFLogxK

U2 - 10.1074/jbc.M608760200

DO - 10.1074/jbc.M608760200

M3 - Article

C2 - 16990268

AN - SCOPUS:33846031944

VL - 281

SP - 37111

EP - 37116

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 48

ER -