D-Titin: A giant protein with dual roles in chromosomes and muscles

Cristina Machado, Deborah J Andrew

Research output: Contribution to journalArticle

Abstract

Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH2-terminal half of vertebrate titin. Mutations in D-Titin cause chromosome undercondensation, chromosome breakage, loss of diploidy, and premature sister chromatid separation. Additionally, D-Titin mutants have defects in myoblast fusion and muscle organization. The phenotypes of the D-Titin mutants suggest parallel roles for titin in both muscle and chromosome structure and elasticity, and provide new insight into chromosome structure.

Original languageEnglish (US)
Pages (from-to)639-651
Number of pages13
JournalJournal of Cell Biology
Volume151
Issue number3
DOIs
StatePublished - Oct 30 2000

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Connectin
Chromosomes
Muscles
Proteins
Chromosome Structures
Chromosome Breakage
Sarcomeres
Chromatids
Myoblasts
Elasticity
Drosophila melanogaster
Diploidy
Vertebrates
Phenotype
Mutation

Keywords

  • Chromosome condensation
  • Drosophila melanogaster
  • Myoblast fusion
  • Sarcomere
  • Titin

ASJC Scopus subject areas

  • Cell Biology

Cite this

D-Titin : A giant protein with dual roles in chromosomes and muscles. / Machado, Cristina; Andrew, Deborah J.

In: Journal of Cell Biology, Vol. 151, No. 3, 30.10.2000, p. 639-651.

Research output: Contribution to journalArticle

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