TY - JOUR
T1 - D-Titin
T2 - A giant protein with dual roles in chromosomes and muscles
AU - Machado, Cristina
AU - Andrew, Deborah J.
PY - 2000/10/30
Y1 - 2000/10/30
N2 - Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH2-terminal half of vertebrate titin. Mutations in D-Titin cause chromosome undercondensation, chromosome breakage, loss of diploidy, and premature sister chromatid separation. Additionally, D-Titin mutants have defects in myoblast fusion and muscle organization. The phenotypes of the D-Titin mutants suggest parallel roles for titin in both muscle and chromosome structure and elasticity, and provide new insight into chromosome structure.
AB - Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH2-terminal half of vertebrate titin. Mutations in D-Titin cause chromosome undercondensation, chromosome breakage, loss of diploidy, and premature sister chromatid separation. Additionally, D-Titin mutants have defects in myoblast fusion and muscle organization. The phenotypes of the D-Titin mutants suggest parallel roles for titin in both muscle and chromosome structure and elasticity, and provide new insight into chromosome structure.
KW - Chromosome condensation
KW - Drosophila melanogaster
KW - Myoblast fusion
KW - Sarcomere
KW - Titin
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U2 - 10.1083/jcb.151.3.639
DO - 10.1083/jcb.151.3.639
M3 - Article
C2 - 11062264
AN - SCOPUS:0034735537
SN - 0021-9525
VL - 151
SP - 639
EP - 651
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
ER -