D-aspartate disposition in neuronal and endocrine tissues

Ontogeny, biosynthesis and release

H. Wolosker, A. D'Aniello, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

High levels of D-aspartate occur in the brain and endocrine glands, such as pineal, adrenal and pituitary. In the brain, D-aspartate levels are highest in embryonic and early postnatal stages. Notably high levels occur in the early postnatal cortical plate and subventricular zone of the cerebral cortical cultures, implying a role in development. In embryonic neuronal primary culture cells, we detected high levels of endogenous D-aspartate and demonstrated biosynthesis of [14C]D-aspartate using [14C]L-aspartate as precursor. Synthesis of D-aspartate in cell cultures is inhibited by amino-oxyacetic acid, an inhibitor of pyridoxal phosphate-dependent enzymes. In the rat adrenal medulla, D-aspartate is depleted by treatment of the animals with intraperitoneal nicotine injections. In adrenal slices, D-aspartate is released by depolarization with KCl or acetylcholine, implying physiological release by activation of the cholinergic innervation of the adrenal.Our characterization of D-aspartate ontogeny, biosynthesis and depolarization-induced release implies specific physiological roles for this amino acid. Copyright (C) 2000 IBRO.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalNeuroscience
Volume100
Issue number1
DOIs
StatePublished - Sep 7 2000

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D-Aspartic Acid
Endocrine Glands
Amino Acids
Pyridoxal Phosphate
Adrenal Medulla
Primary Cell Culture
Lateral Ventricles
Brain
Intraperitoneal Injections
Nicotine
Aspartic Acid
Cerebral Cortex
Cholinergic Agents
Acetylcholine
Cell Culture Techniques
Enzymes

Keywords

  • Adrenal medulla
  • D-amino acid oxidase
  • D-amino acids
  • D-aspartate oxidase
  • D-serine
  • Pineal gland

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

D-aspartate disposition in neuronal and endocrine tissues : Ontogeny, biosynthesis and release. / Wolosker, H.; D'Aniello, A.; Snyder, Solomon H.

In: Neuroscience, Vol. 100, No. 1, 07.09.2000, p. 183-189.

Research output: Contribution to journalArticle

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