Abstract
High levels of D-aspartate occur in the brain and endocrine glands, such as pineal, adrenal and pituitary. In the brain, D-aspartate levels are highest in embryonic and early postnatal stages. Notably high levels occur in the early postnatal cortical plate and subventricular zone of the cerebral cortical cultures, implying a role in development. In embryonic neuronal primary culture cells, we detected high levels of endogenous D-aspartate and demonstrated biosynthesis of [14C]D-aspartate using [14C]L-aspartate as precursor. Synthesis of D-aspartate in cell cultures is inhibited by amino-oxyacetic acid, an inhibitor of pyridoxal phosphate-dependent enzymes. In the rat adrenal medulla, D-aspartate is depleted by treatment of the animals with intraperitoneal nicotine injections. In adrenal slices, D-aspartate is released by depolarization with KCl or acetylcholine, implying physiological release by activation of the cholinergic innervation of the adrenal.Our characterization of D-aspartate ontogeny, biosynthesis and depolarization-induced release implies specific physiological roles for this amino acid. Copyright (C) 2000 IBRO.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-189 |
| Number of pages | 7 |
| Journal | Neuroscience |
| Volume | 100 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 7 2000 |
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Keywords
- Adrenal medulla
- D-amino acid oxidase
- D-amino acids
- D-aspartate oxidase
- D-serine
- Pineal gland
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
D-aspartate disposition in neuronal and endocrine tissues : Ontogeny, biosynthesis and release. / Wolosker, H.; D'Aniello, A.; Snyder, Solomon H.
In: Neuroscience, Vol. 100, No. 1, 07.09.2000, p. 183-189.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - D-aspartate disposition in neuronal and endocrine tissues
T2 - Ontogeny, biosynthesis and release
AU - Wolosker, H.
AU - D'Aniello, A.
AU - Snyder, Solomon H
PY - 2000/9/7
Y1 - 2000/9/7
N2 - High levels of D-aspartate occur in the brain and endocrine glands, such as pineal, adrenal and pituitary. In the brain, D-aspartate levels are highest in embryonic and early postnatal stages. Notably high levels occur in the early postnatal cortical plate and subventricular zone of the cerebral cortical cultures, implying a role in development. In embryonic neuronal primary culture cells, we detected high levels of endogenous D-aspartate and demonstrated biosynthesis of [14C]D-aspartate using [14C]L-aspartate as precursor. Synthesis of D-aspartate in cell cultures is inhibited by amino-oxyacetic acid, an inhibitor of pyridoxal phosphate-dependent enzymes. In the rat adrenal medulla, D-aspartate is depleted by treatment of the animals with intraperitoneal nicotine injections. In adrenal slices, D-aspartate is released by depolarization with KCl or acetylcholine, implying physiological release by activation of the cholinergic innervation of the adrenal.Our characterization of D-aspartate ontogeny, biosynthesis and depolarization-induced release implies specific physiological roles for this amino acid. Copyright (C) 2000 IBRO.
AB - High levels of D-aspartate occur in the brain and endocrine glands, such as pineal, adrenal and pituitary. In the brain, D-aspartate levels are highest in embryonic and early postnatal stages. Notably high levels occur in the early postnatal cortical plate and subventricular zone of the cerebral cortical cultures, implying a role in development. In embryonic neuronal primary culture cells, we detected high levels of endogenous D-aspartate and demonstrated biosynthesis of [14C]D-aspartate using [14C]L-aspartate as precursor. Synthesis of D-aspartate in cell cultures is inhibited by amino-oxyacetic acid, an inhibitor of pyridoxal phosphate-dependent enzymes. In the rat adrenal medulla, D-aspartate is depleted by treatment of the animals with intraperitoneal nicotine injections. In adrenal slices, D-aspartate is released by depolarization with KCl or acetylcholine, implying physiological release by activation of the cholinergic innervation of the adrenal.Our characterization of D-aspartate ontogeny, biosynthesis and depolarization-induced release implies specific physiological roles for this amino acid. Copyright (C) 2000 IBRO.
KW - Adrenal medulla
KW - D-amino acid oxidase
KW - D-amino acids
KW - D-aspartate oxidase
KW - D-serine
KW - Pineal gland
UR - http://www.scopus.com/inward/record.url?scp=0034618848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034618848&partnerID=8YFLogxK
U2 - 10.1016/S0306-4522(00)00321-3
DO - 10.1016/S0306-4522(00)00321-3
M3 - Article
VL - 100
SP - 183
EP - 189
JO - Neuroscience
JF - Neuroscience
SN - 0306-4522
IS - 1
ER -