d-Aspartate and d-aspartate oxidase show selective and developmentally dynamic localization in mouse retina

Alex S. Huang, Dan A. Lee, Seth Blackshaw

Research output: Contribution to journalArticlepeer-review

Abstract

d-Enantiomer amino acids, endogenously synthesized and degraded in mammals, participate in multiple developmental and physiological processes. We characterize both d-aspartate and its degradative enzyme (d-aspartate oxidase) in post-natal mouse retina. d-Aspartate attains a developmental peak of 886 nmol/g dry weight at 1-2 weeks post-natal while l-aspartate transiently declines, consistent with the de novo synthesis of d-aspartate. d-Aspartate is localized in many cell-types in different retinal layers, with a notable shift from outer retina to inner retina with time. d-Aspartate oxidase is localized to horizontal cells by in situ hybridization, immunohistochemistry, and histochemical staining methods. Given that final cell specification, differentiation, and synaptogenesis occur during the immediate 2-week post-natal period in the rodent retina and the dynamic appearance of d-aspartate and d-aspartate oxidase, we propose that d-aspartate plays a role in mammalian retinal development.

Original languageEnglish (US)
Pages (from-to)704-709
Number of pages6
JournalExperimental eye research
Volume86
Issue number4
DOIs
StatePublished - Apr 2008

Keywords

  • d-aspartate
  • d-aspartate oxidase
  • development
  • retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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