Cytotoxicity assay in antibiotic-associated colitis

Te Wen Chang, Michael Lauermann, John Bartlett

Research output: Contribution to journalArticle

Abstract

Stools were tested for the presence of a cytopathic toxin that is neutralized by gas gangrene antitoxin or Clostridium sordellii antitoxin. The test was positive in specimens from 51 (96%) of 53 patients with antibiotic-associated pseudomembranous colitis and five (2%) of 248 individuals without gastrointestinal complications of antimicrobial usage. Seven types of tissue cultures were tested, and all proved susceptible to this toxin. Those that appeared to be the most susceptible on the basis of titers of toxin in stools were primary human amnion cells, WI-38 cells, baby hamster kidney cells, and mouse fibroblasts. Storage of specimens at-70 C, 6 C, 28 C, and 37 C for five days before testing resulted in decrease in toxin titers that correlated with increasing temperatures. Studies of various C. sordellii antitoxin preparations indicated considerable variation in potency. Recommendations for performing the tissue culture assay for the toxin found in stools of patients with antibiotic-associated clitis are provided on the basis of these observations.

Original languageEnglish (US)
Pages (from-to)765-770
Number of pages6
JournalJournal of Infectious Diseases
Volume140
Issue number5
DOIs
StatePublished - 1979
Externally publishedYes

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Pseudomembranous Enterocolitis
Antitoxins
Clostridium sordellii
Gas Gangrene
Amnion
Cricetinae
Fibroblasts
Anti-Bacterial Agents
Kidney
Temperature

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Immunology
  • Public Health, Environmental and Occupational Health

Cite this

Cytotoxicity assay in antibiotic-associated colitis. / Chang, Te Wen; Lauermann, Michael; Bartlett, John.

In: Journal of Infectious Diseases, Vol. 140, No. 5, 1979, p. 765-770.

Research output: Contribution to journalArticle

Chang, Te Wen ; Lauermann, Michael ; Bartlett, John. / Cytotoxicity assay in antibiotic-associated colitis. In: Journal of Infectious Diseases. 1979 ; Vol. 140, No. 5. pp. 765-770.
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