Cytotoxic enterotoxin produced by Aeromonas hydrophila: Relationship of toxigenic isolates to diarrheal disease

N. Cumberbatch, M. J. Gurwith, C. Langston, R. B. Sack, J. L. Brunton

Research output: Contribution to journalArticle

Abstract

Ninety-six Aeromonas hydrophila isolates were tested for cytotoxin and hemolysin production. Sixty-six (69%) of the isolates were both cytotoxic and hemolytic, whereas the rest produced neither cytotoxin nor hemolysin. No evidence of a separate cytotonic activity could be found in any of the isolates. Cytotoxin activity correlated with enterotoxic activity. Of four cytotoxin-producing strains tested in the isolated rabbit ileal loop, three were definitely positive and one was borderline, whereas two nontoxigenic strains were negative. Cytotoxin activity appeared to be a stable property and could not be associated with any common identified plasmid; only 10 of 21 cytotoxin-producing strains could be shown to have any plasmid by agarose gel electrophoresis. Cytotoxin production correlated with a positive lysine decarboxylase phenotype (98%) or a positive Voges-Proskauer phenotype (94%), compared to 27% lysine decarboxylase-positive and 23% Voges-zProskauer-positive, cytotoxin-negative isolates (P

Original languageEnglish (US)
Pages (from-to)829-837
Number of pages9
JournalInfection and Immunity
Volume23
Issue number3
StatePublished - 1979
Externally publishedYes

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Aeromonas hydrophila
Enterotoxins
Cytotoxins
lysine decarboxylase
Hemolysin Proteins
Plasmids
Phenotype
Agar Gel Electrophoresis
Rabbits

ASJC Scopus subject areas

  • Immunology

Cite this

Cumberbatch, N., Gurwith, M. J., Langston, C., Sack, R. B., & Brunton, J. L. (1979). Cytotoxic enterotoxin produced by Aeromonas hydrophila: Relationship of toxigenic isolates to diarrheal disease. Infection and Immunity, 23(3), 829-837.

Cytotoxic enterotoxin produced by Aeromonas hydrophila : Relationship of toxigenic isolates to diarrheal disease. / Cumberbatch, N.; Gurwith, M. J.; Langston, C.; Sack, R. B.; Brunton, J. L.

In: Infection and Immunity, Vol. 23, No. 3, 1979, p. 829-837.

Research output: Contribution to journalArticle

Cumberbatch, N, Gurwith, MJ, Langston, C, Sack, RB & Brunton, JL 1979, 'Cytotoxic enterotoxin produced by Aeromonas hydrophila: Relationship of toxigenic isolates to diarrheal disease', Infection and Immunity, vol. 23, no. 3, pp. 829-837.
Cumberbatch, N. ; Gurwith, M. J. ; Langston, C. ; Sack, R. B. ; Brunton, J. L. / Cytotoxic enterotoxin produced by Aeromonas hydrophila : Relationship of toxigenic isolates to diarrheal disease. In: Infection and Immunity. 1979 ; Vol. 23, No. 3. pp. 829-837.
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