Cytotoxic effects of N-(3-chloro-1,4-dioxo 1,4-dihydro-naphthalen-2-yl)- benzamide on androgen-dependent and -independent prostate cancer cell lines

Yasmine M. Kanaan, Douglas F. White, Jharna R. Das, Solomon Berhe, Oladapo Bakare, Hillaire Kenguele, Desta Beyene, Yanfei Zhou, Agnes A. Day, Robert L. Copeland

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Worldwide among men, prostate cancer ranks third in cancer occurrence and sixth in cancer mortality. A number of 1, 4-naphthoquinone derivatives have been identified that possess significant pharmacological effects associated with antitumor activities. In this study, the in vitro effects of N-(3-chloro-1,4-dioxo 1,4-dihydro-naphthalen-2-yl)-benzamide (NCDDNB) were evaluated on androgen-dependent (CWR-22) and androgen-independent (PC-3, DU-145) human prostate cancer cell lines, and on a normal bone marrow cell line (HS-5). Specifically, the in vitro activity of this compound on cell cycle regulation and apoptosis was evaluated. Materials and Methods: Established methods of cell viability, cell cycle, Western blot and apoptosis were used. Results: The effect of NCDDNB on CWR-22, PC-3, DU-145 and HS-5 cells revealed significant anti-tumor activities with IC50s, of 2.5, 2.5, 6.5, and 25 μM respectively. The results of cell cycle analysis showed that NCDDNB arrested PC-3, DU-145, and CWR-22 cells in the G1-phase of the cell cycle. The compound showed no effect on the cell cycle progression in the HS-5 bone marrow cell line. These findings were further validated using Western blot analysis. NCDDNB showed the greatest amount of apoptosis in the androgen-independent PC-3 cells in a time-dependent manner with the apoptotic apex at day 5 of treatment.

Original languageEnglish (US)
Pages (from-to)519-527
Number of pages9
JournalAnticancer Research
Volume30
Issue number2
StatePublished - Feb 2010
Externally publishedYes

Keywords

  • 1,4-naphthoquinone
  • Apoptosis
  • Cell cycle
  • Cytotoxicity
  • Human prostate cancer cell lines

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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