TY - JOUR
T1 - Cytotoxic effects of anticancer agents on subconfluent and multilayered postconfluent cultures
AU - Pizao, Paulo E.
AU - Peters, Godefridus J.
AU - Ark-Otte, Jannette Van
AU - Smets, Lou A.
AU - Smitskamp-Wilms, Evelien
AU - Winograd, Benjamin
AU - Pinedo, Herbert M.
AU - Giaccone, Giuseppe
PY - 1993
Y1 - 1993
N2 - The cytotoxic effects of conventional (doxorubicin, 5-fluorouracil, cisplatin) and investigational (2′,2′-difluorodeoxycytidine, hexadecylphosphocholine, EO9, rhizoxin) anticancer drugs were studied in subconfluent and multilayered postconfluent cultures of human colon and ovarian carcinoma cell lines. Chemosensitivity was assessed 4 days after a 24-h drug exposure with the sulphorhodamine B assay. Except for rhizoxin, all drugs tested yielded an ec50 (drug concentration producing absorbance readings 50% lower than those of non-treated wells) in postconfluent cultures that were higher than an ec50 obtained with subconfluent cultures. Compared with subconfluent cultures, postconfluent cultures showed decreased cellular nucleotide concentrations and ATP/ADP ratios, in addition to an increased percentage of G0/G1 activity of DT-diaphorase, a reductase involved in the bioactivation of EO9, was similar in sub- and postconfluent cultures. These results indicate similarity of the postconfluent model presented with those obtained with in vivo models and more complex in vitro techniques.
AB - The cytotoxic effects of conventional (doxorubicin, 5-fluorouracil, cisplatin) and investigational (2′,2′-difluorodeoxycytidine, hexadecylphosphocholine, EO9, rhizoxin) anticancer drugs were studied in subconfluent and multilayered postconfluent cultures of human colon and ovarian carcinoma cell lines. Chemosensitivity was assessed 4 days after a 24-h drug exposure with the sulphorhodamine B assay. Except for rhizoxin, all drugs tested yielded an ec50 (drug concentration producing absorbance readings 50% lower than those of non-treated wells) in postconfluent cultures that were higher than an ec50 obtained with subconfluent cultures. Compared with subconfluent cultures, postconfluent cultures showed decreased cellular nucleotide concentrations and ATP/ADP ratios, in addition to an increased percentage of G0/G1 activity of DT-diaphorase, a reductase involved in the bioactivation of EO9, was similar in sub- and postconfluent cultures. These results indicate similarity of the postconfluent model presented with those obtained with in vivo models and more complex in vitro techniques.
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U2 - 10.1016/0959-8049(93)90296-R
DO - 10.1016/0959-8049(93)90296-R
M3 - Article
C2 - 8217364
AN - SCOPUS:0027268665
SN - 0959-8049
VL - 29
SP - 1566
EP - 1573
JO - European Journal of Cancer
JF - European Journal of Cancer
IS - 11
ER -