Cytotoxic effects of anticancer agents on subconfluent and multilayered postconfluent cultures

Paulo E. Pizao, Godefridus J. Peters, Jannette Van Ark-Otte, Lou A. Smets, Evelien Smitskamp-Wilms, Benjamin Winograd, Herbert M. Pinedo, Giuseppe Giaccone

Research output: Contribution to journalArticle

Abstract

The cytotoxic effects of conventional (doxorubicin, 5-fluorouracil, cisplatin) and investigational (2′,2′-difluorodeoxycytidine, hexadecylphosphocholine, EO9, rhizoxin) anticancer drugs were studied in subconfluent and multilayered postconfluent cultures of human colon and ovarian carcinoma cell lines. Chemosensitivity was assessed 4 days after a 24-h drug exposure with the sulphorhodamine B assay. Except for rhizoxin, all drugs tested yielded an ec50 (drug concentration producing absorbance readings 50% lower than those of non-treated wells) in postconfluent cultures that were higher than an ec50 obtained with subconfluent cultures. Compared with subconfluent cultures, postconfluent cultures showed decreased cellular nucleotide concentrations and ATP/ADP ratios, in addition to an increased percentage of G0/G1 activity of DT-diaphorase, a reductase involved in the bioactivation of EO9, was similar in sub- and postconfluent cultures. These results indicate similarity of the postconfluent model presented with those obtained with in vivo models and more complex in vitro techniques.

Original languageEnglish (US)
Pages (from-to)1566-1573
Number of pages8
JournalEuropean Journal of Cancer
Volume29
Issue number11
DOIs
Publication statusPublished - 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Pizao, P. E., Peters, G. J., Ark-Otte, J. V., Smets, L. A., Smitskamp-Wilms, E., Winograd, B., ... Giaccone, G. (1993). Cytotoxic effects of anticancer agents on subconfluent and multilayered postconfluent cultures. European Journal of Cancer, 29(11), 1566-1573. https://doi.org/10.1016/0959-8049(93)90296-R