Cytotoxic and cell vacuolating activity of Vibrio fluvialis isolated from paediatric patients with diarrhoea

Rupa Chakraborty, Subhra Chakraborty, Keya De, Sutapa Sinha, Asish K. Mukhopadhyay, Jasmina Khanam, Thandavarayan Ramamurthy, Yoshifumi Takeda, Sujit K. Bhattacharya, G. Balakrish Nair

Research output: Contribution to journalArticlepeer-review

Abstract

Vibrio fluvialis is a halophilic Vibrio species associated with acute diarrhoeal illness in humans. It has the potential to cause outbreaks and has an association with paediatric diarrhoea. In this study, 11 V. fluvialis strains isolated from hospitalized patients with acute diarrhoea at the Infectious Diseases Hospital, Kolkata were extensively characterized. All the strains showed growth in peptone broth containing 7% NaCl. The strains showed variable results in Voges-Proskauer test and to a vibriostatic agent. There was also variation in their antibiograms, and some of the strains were multidrug resistant. Among the 11 strains, two showed only a single band difference in their PFGE profile and the remaining strains showed nine different PFGE patterns. However, unlike PFGE, the strains exhibited close matches and clustering in their ribotype patterns. The haemolytic effect on sheep red blood cells varied with strains. Partial sequence analysis revealed that the V. fluvialis haemolysin gene has 81% homology with that of the El Tor haemolysin of Vibrio cholerae. A striking finding was the capability of all the strains to evoke distinct cytotoxic and vacuolation effects on HeLa cells.

Original languageEnglish (US)
Pages (from-to)707-716
Number of pages10
JournalJournal of medical microbiology
Volume54
Issue number8
DOIs
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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