Cytosolic proteostasis through importing of misfolded proteins into mitochondria

Linhao Ruan, Chuankai Zhou, Erli Jin, Andrei Kucharavy, Ying Zhang, Zhihui Wen, Laurence Florens, Rong Li

Research output: Contribution to journalArticle

Abstract

Loss of proteostasis underlies ageing and neurodegeneration characterized by the accumulation of protein aggregates and mitochondrial dysfunction. Although many neurodegenerative-disease-associated proteins can be found in mitochondria, it remains unclear how mitochondrial dysfunction and protein aggregation could be related. In dividing yeast cells, protein aggregates that form under stress or during ageing are preferentially retained by the mother cell, in part through tethering to mitochondria, while the disaggregase Hsp104 helps to dissociate aggregates and thereby enables refolding or degradation of misfolded proteins. Here we show that, in yeast, cytosolic proteins prone to aggregation are imported into mitochondria for degradation. Protein aggregates that form under heat shock contain both cytosolic and mitochondrial proteins and interact with the mitochondrial import complex. Many aggregation-prone proteins enter the mitochondrial intermembrane space and matrix after heat shock, and some do so even without stress. Timely dissolution of cytosolic aggregates requires the mitochondrial import machinery and proteases. Blocking mitochondrial import but not proteasome activity causes a marked delay in the degradation of aggregated proteins. Defects in cytosolic Hsp70s leads to enhanced entry of misfolded proteins into mitochondria and elevated mitochondrial stress. We term this mitochondria-mediated proteostasis mechanism MAGIC (mitochondria as guardian in cytosol) and provide evidence that it may exist in human cells.

Original languageEnglish (US)
Pages (from-to)443-446
Number of pages4
JournalNature
Volume543
Issue number7645
DOIs
StatePublished - Mar 16 2017

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Mitochondria
Mitochondrial Proteins
Proteins
Fungal Proteins
Proteolysis
Shock
Hot Temperature
Proteasome Endopeptidase Complex
Neurodegenerative Diseases
Cytosol
Peptide Hydrolases
Stem Cells
Protein Aggregates

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Ruan, L., Zhou, C., Jin, E., Kucharavy, A., Zhang, Y., Wen, Z., ... Li, R. (2017). Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Nature, 543(7645), 443-446. https://doi.org/10.1038/nature21695

Cytosolic proteostasis through importing of misfolded proteins into mitochondria. / Ruan, Linhao; Zhou, Chuankai; Jin, Erli; Kucharavy, Andrei; Zhang, Ying; Wen, Zhihui; Florens, Laurence; Li, Rong.

In: Nature, Vol. 543, No. 7645, 16.03.2017, p. 443-446.

Research output: Contribution to journalArticle

Ruan, L, Zhou, C, Jin, E, Kucharavy, A, Zhang, Y, Wen, Z, Florens, L & Li, R 2017, 'Cytosolic proteostasis through importing of misfolded proteins into mitochondria', Nature, vol. 543, no. 7645, pp. 443-446. https://doi.org/10.1038/nature21695
Ruan L, Zhou C, Jin E, Kucharavy A, Zhang Y, Wen Z et al. Cytosolic proteostasis through importing of misfolded proteins into mitochondria. Nature. 2017 Mar 16;543(7645):443-446. https://doi.org/10.1038/nature21695
Ruan, Linhao ; Zhou, Chuankai ; Jin, Erli ; Kucharavy, Andrei ; Zhang, Ying ; Wen, Zhihui ; Florens, Laurence ; Li, Rong. / Cytosolic proteostasis through importing of misfolded proteins into mitochondria. In: Nature. 2017 ; Vol. 543, No. 7645. pp. 443-446.
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