Cytoskeleton-interacting LIM-domain protein CRP1 suppresses cell proliferation and protects from stress-induced cell death

Leena Latonen, Päivi M. Järvinen, Marikki Laiho

Research output: Contribution to journalArticlepeer-review

Abstract

Members of the cysteine-rich protein (CRP) family are actin cytoskeleton-interacting LIM-domain proteins known to act in muscle cell differentiation. We have earlier found that CRP1, a founding member of this family, is transcriptionally induced by UV radiation in human diploid fibroblasts [M. Gentile, L. Latonen, M. Laiho, Cell cycle arrest and apoptosis provoked by UV radiation-induced DNA damage are transcriptionally highly divergent responses, Nucleic Acids Res. 31 (2003) 4779-4790]. Here we show that CRP1 is induced by growth-inhibitory signals, such as increased cellular density, and cytotoxic stress induced by UV radiation or staurosporine. We found that high levels of CRP1 correlate with differentiation-associated morphology towards the myofibroblast lineage and that expression of ectopic CRP1 suppresses cell proliferation. Following UV- and staurosporine-induced stresses, expression of CRP1 provides a survival advantage evidenced by decreased cellular death and increased cellular metabolic activity and attachment. Our studies identify that CRP1 is a novel stress response factor, and provide evidence for its growth-inhibitory and cytoprotective functions.

Original languageEnglish (US)
Pages (from-to)738-747
Number of pages10
JournalExperimental cell research
Volume314
Issue number4
DOIs
StatePublished - Feb 15 2008
Externally publishedYes

Keywords

  • Apoptosis
  • Cellular stress
  • Cytoskeleton
  • Growth inhibition
  • LIM-domain
  • Staurosporine
  • UV radiation

ASJC Scopus subject areas

  • Cell Biology

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