TY - JOUR
T1 - Cytophotometric analyses of mesencephalic reticular formation neuronal RNA in soman intoxicated rats
AU - Doebler, J. A.
AU - Martin, L. J.
AU - Shih, T. M.
AU - Anthony, A.
PY - 1985
Y1 - 1985
N2 - Quantitative azure B cytophotometry was used to monitor ribonucleic acid (RNA) responses of individual neurons within the nucleus cuneiformis (NC) and ventrotegmental nucleus (VTN) of the rat mesencephalic reticular formation following single subcutaneous soman (pinacolyl methylphosphonofluoridate) injections (0.5, 0.9 or 1.5 LD50). The sub-lethal (0.5 LD50) dosage of soman produced RNA accumulation in NC neurons, but VTN-RNA levels were not significantly altered. In contrast, both reticular nuclei exhibited prominent RNA depletion with higher soman dosages, the severity of which was greater with lethal (1.5 LD50) than near-lethal (0.9 LD50) dosages. These data indicate that metabolic correlates of enhanced activation of cholinergic reticular nuclei are present only with sub-lethal dosages, and that higher dosages produce characteristics of impaired activation of ascending cholinergic pathways. At present, mechanisms underlying soman-induced metabolic and neurologic deficits remain speculative.
AB - Quantitative azure B cytophotometry was used to monitor ribonucleic acid (RNA) responses of individual neurons within the nucleus cuneiformis (NC) and ventrotegmental nucleus (VTN) of the rat mesencephalic reticular formation following single subcutaneous soman (pinacolyl methylphosphonofluoridate) injections (0.5, 0.9 or 1.5 LD50). The sub-lethal (0.5 LD50) dosage of soman produced RNA accumulation in NC neurons, but VTN-RNA levels were not significantly altered. In contrast, both reticular nuclei exhibited prominent RNA depletion with higher soman dosages, the severity of which was greater with lethal (1.5 LD50) than near-lethal (0.9 LD50) dosages. These data indicate that metabolic correlates of enhanced activation of cholinergic reticular nuclei are present only with sub-lethal dosages, and that higher dosages produce characteristics of impaired activation of ascending cholinergic pathways. At present, mechanisms underlying soman-induced metabolic and neurologic deficits remain speculative.
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U2 - 10.1016/0197-0186(85)90163-9
DO - 10.1016/0197-0186(85)90163-9
M3 - Article
AN - SCOPUS:0021875765
SN - 0197-0186
VL - 7
SP - 415
EP - 420
JO - Neurochemistry International
JF - Neurochemistry International
IS - 3
ER -