TY - JOUR
T1 - Cytomegalovirus Kinetics Following Primary Infection in Healthy Women
AU - Forman, Michael S.
AU - Vaidya, Dhananjay
AU - Bolorunduro, Oluwaseyi
AU - Diener-West, Marie
AU - Pass, Robert F.
AU - Arav-Boger, Ravit
N1 - Funding Information:
This work was supported by the National Institutes of Health (grant numbers KO8 1K08AI074907 to R. A. B. and U01-AI063565 to R. F. P.). The statistical analysis was supported by the Johns Hopkins Institute for Clinical and Translational Research (ICTR), funded in part by the National Center for Advancing Translational Sciences (NCATS), Grant Number UL1 TR001079.
Publisher Copyright:
© The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved.
PY - 2017/5/15
Y1 - 2017/5/15
N2 - The kinetics of cytomegalovirus (CMV) DNA in infected asymptomatic hosts are largely unknown. We measured viral load (VL) in 124 fluid samples (oral, urine, vaginal, blood) collected from 21 women who acquired CMV. A quantitative real-time polymerase chain reaction assay of US17, which correlated with clinical assays, was used. VL decreased following primary infection in all fluids. The geometric mean VL of vaginal fluid was significantly higher than that of other sources: oral (3.89; 95% confidence interval [CI], 1.43-10.57), urine (6.36; 95% CI, 2.48-16.32), and whole blood (11.88; 95% CI, 4.12-34.20). Vaginal CMV shedding may provide a route for sexual and possibly perinatal transmission.
AB - The kinetics of cytomegalovirus (CMV) DNA in infected asymptomatic hosts are largely unknown. We measured viral load (VL) in 124 fluid samples (oral, urine, vaginal, blood) collected from 21 women who acquired CMV. A quantitative real-time polymerase chain reaction assay of US17, which correlated with clinical assays, was used. VL decreased following primary infection in all fluids. The geometric mean VL of vaginal fluid was significantly higher than that of other sources: oral (3.89; 95% confidence interval [CI], 1.43-10.57), urine (6.36; 95% CI, 2.48-16.32), and whole blood (11.88; 95% CI, 4.12-34.20). Vaginal CMV shedding may provide a route for sexual and possibly perinatal transmission.
KW - Cytomegalovirus
KW - US17 real-time PCR
KW - normal host
KW - viral loads
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U2 - 10.1093/infdis/jix188
DO - 10.1093/infdis/jix188
M3 - Article
C2 - 28431127
AN - SCOPUS:85021829857
SN - 0022-1899
VL - 215
SP - 1523
EP - 1526
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 10
ER -