Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥7.0 log10 copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤2.1% at 3.0 and 5.0 log10 copies/ml. In clinical specimens, RGQ values were ∼0.2 log10 copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.
ASJC Scopus subject areas
- Microbiology (medical)