TY - JOUR
T1 - Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay setup
AU - Forman, Michael
AU - Wilson, Andy
AU - Valsamakis, Alexandra
PY - 2011/7
Y1 - 2011/7
N2 - Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥7.0 log10 copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤2.1% at 3.0 and 5.0 log10 copies/ml. In clinical specimens, RGQ values were ∼0.2 log10 copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.
AB - Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥7.0 log10 copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤2.1% at 3.0 and 5.0 log10 copies/ml. In clinical specimens, RGQ values were ∼0.2 log10 copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.
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U2 - 10.1128/JCM.00721-11
DO - 10.1128/JCM.00721-11
M3 - Article
C2 - 21562101
AN - SCOPUS:79959815329
SN - 0095-1137
VL - 49
SP - 2703
EP - 2705
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 7
ER -