Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay setup

Michael Forman, Andy Wilson, Alexandra Valsamakis

Research output: Contribution to journalArticle

Abstract

Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥7.0 log10 copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤2.1% at 3.0 and 5.0 log10 copies/ml. In clinical specimens, RGQ values were ∼0.2 log10 copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.

Original languageEnglish (US)
Pages (from-to)2703-2705
Number of pages3
JournalJournal of clinical microbiology
Volume49
Issue number7
DOIs
StatePublished - Jul 1 2011

ASJC Scopus subject areas

  • Microbiology (medical)

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