Cytomegalovirus basic phosphoprotein (pUL32) binds to capsids in vitro through its amino one-third

M. K. Baxter, D Wade Gibson

Research output: Contribution to journalArticle

Abstract

The cytomegalovirus (CMV) basic phosphoprotein (BPP) is a component of the tegument. It remains with the nucleocapsid fraction under conditions that remove most other tegument proteins from the virion, suggesting a direct and perhaps tight interaction with the capsid. AS a step toward localizing this protein within the molecular structure of the virion and understanding its function during infection, we have investigated the BPP-capsid interaction. In this report we present evidence that the BPP interacts selectively, through its amino one-third, with CMV capsids. Radiolabeled simian CMV (SCMV) BPP, synthesized in vitro, bound to SCMV B-capsids, and C-capsids to a lesser extent, following incubation with either isolated capsids or lysates of infected cells. Human CMV (HCMV) BPP (pUL32) also bound to SCMV capsids, and SCMV BPP likewise bound to HCMV capsids, indicating that the sequence(s) involved is conserved between the two proteins. Analysis of SCMV BPP truncation mutants localized the capsid-binding region to the amino one-third of the molecule - the portion of BPP showing the greatest sequence conservation between the SCMV and HCMV homologs. This general approach may have utility in studying the interactions of other proteins with conformation-dependent binding sites.

Original languageEnglish (US)
Pages (from-to)6865-6873
Number of pages9
JournalJournal of Virology
Volume75
Issue number15
DOIs
StatePublished - 2001

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Cytomegalovirus
phosphoproteins
capsid
Capsid
Phosphoproteins
virion
Virion
proteins
Human herpesvirus 5
In Vitro Techniques
Nucleocapsid
nucleocapsid
Protein Conformation
Proteins
Molecular Structure
chemical structure
binding sites
Binding Sites
mutants

ASJC Scopus subject areas

  • Immunology

Cite this

Cytomegalovirus basic phosphoprotein (pUL32) binds to capsids in vitro through its amino one-third. / Baxter, M. K.; Gibson, D Wade.

In: Journal of Virology, Vol. 75, No. 15, 2001, p. 6865-6873.

Research output: Contribution to journalArticle

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