Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis

K. Persson Waller, I. G. Colditz, S. Lun, K. Östensson

Research output: Contribution to journalArticlepeer-review

Abstract

Cytokine kinetics were examined in milk and in afferent and efferent lymph of the supramammary lymph node after intramammary infusion of endotoxin from Escherichia coli. Cows were sampled 0, 2 and 4 h after infusion (p.i.). Neutrophils appeared in afferent lymph 2 h p.i., and in efferent lymph and milk 4 h p.i. The milk contained high concentrations of interleukin-8 (IL-8) at 2 and 4 h p.i. IL-8 was also found in lymph, but at lower concentrations. The tumor necrosis factor-α (TNF-α) concentration tended to increase in afferent lymph at 2 h p.i., and increased in milk at 4 h p.i. The level of IL-1β increased at 4 h p.i. in milk, but was not detected in lymph. Interferon-γ was not detected in any sample, at any time. The results indicate a primary role for IL-8 in the recruitment of neutrophils into the gland, and suggest that IL-1β and TNF-α are not necessary for IL-8 production and release in response to endotoxin.

Original languageEnglish (US)
Pages (from-to)31-36
Number of pages6
JournalResearch in Veterinary Science
Volume74
Issue number1
DOIs
StatePublished - Feb 2003
Externally publishedYes

ASJC Scopus subject areas

  • veterinary(all)

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