Cytokine production by bone marrow mononuclear cells in inherited bone marrow failure syndromes

Ken Matsui, Neelam Giri, Blanche P. Alter, Ligia A. Pinto

Research output: Contribution to journalArticle

Abstract

Fanconi anaemia (FA), dyskeratosis congenita (DC), Diamond-Blackfan anaemia (DBA), and Shwachman-Diamond syndrome (SDS) are characterized by the progressive development of bone marrow failure. Overproduction of tumour necrosis factor-α (TNF-α) from activated bone marrow T-cells has been proposed as a mechanism of FA-related aplasia. Whether such overproduction occurs in the other syndromes is unknown. We conducted a comparative study on bone marrow mononuclear cells to examine the cellular subset composition and cytokine production. We found lower proportions of haematopoietic stem cells in FA, DC, and SDS, and a lower proportion of monocytes in FA, DC, and DBA compared with controls. The T- and B-lymphocyte proportions were similar to controls, except for low B-cells in DC. We did not observe overproduction of TNF-α or IFN-γ by T-cells in any patients. Induction levels of TNF-α, interleukin (IL)-6, IL-1β, IL-10, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor in monocytes stimulated with high-dose lipopolysaccharide (LPS) were similar at 4 h but lower at 24 h when compared to controls. Unexpectedly, patient samples showed a trend toward higher cytokine level in response to low-dose (0·001 μg/ml) LPS. Increased sensitivity to LPS may have clinical implications and could contribute to the development of pancytopenia by creating a chronic subclinical inflammatory micro-environment in the bone marrow.

Original languageEnglish (US)
Pages (from-to)81-92
Number of pages12
JournalBritish Journal of Haematology
Volume163
Issue number1
DOIs
StatePublished - Oct 2013
Externally publishedYes

Keywords

  • Bone marrow
  • Cytokines
  • Immunology
  • Inherited bone marrow failure syndromes
  • Monocytes

ASJC Scopus subject areas

  • Hematology

Fingerprint Dive into the research topics of 'Cytokine production by bone marrow mononuclear cells in inherited bone marrow failure syndromes'. Together they form a unique fingerprint.

  • Cite this