Cytokine induction of nuclear factor κB in cycling and growth-arrested cells: Evidence for cell cycle-independent activation

Colin S. Duckett, Neil D. Perkins, Kwanyee Leung, Adam B. Agranoff, Gary J. Nabel

Research output: Contribution to journalArticlepeer-review

Abstract

Nuclear factor κB (NF-κB) is a pleiotropic transcription factor which regulates the expression of a large number of cellular and viral genes. Induction of NF-κB has been shown previously to occur during cell cycle transition from G0 to G1, but the relationship of cytokine induction of this transcription factor to cell cycling has not been directly addressed. Here we examine the induction of NF-κB in serum-deprived and cycling cells in response to tumor necrosis factor-α (TNF-α). In 3T3 fibroblasts deprived of serum, and in the temperaturesensitive G2 phase mutant carcinoma line FT210, we find that NF-κB DNA binding activity is rapidly induced upon addition of TNF-α. In addition, NF-κB induction in cycling cells occurs without a significant change in cell cycle distribution. These data reveal that NF-κB is rapidly induced by TNF-α in both proliferating and arrested cells and suggest that distinct activation pathways can lead to cell cycle-dependent or -independent induction of NF-κB.

Original languageEnglish (US)
Pages (from-to)18836-18840
Number of pages5
JournalJournal of Biological Chemistry
Volume270
Issue number32
StatePublished - Aug 11 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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