Cytokine-induced memory-like natural killer cells exhibit enhanced responses against myeloid leukemia

Rizwan Romee, Maxim Rosario, Melissa M. Berrien-Elliott, Julia A. Wagner, Brea A. Jewell, Timothy Schappe, Jeffrey W. Leong, Sara Abdel-Latif, Stephanie E. Schneider, Sarah Willey, Carly C. Neal, Liyang Yu, Stephen T. Oh, Yi Shan Lee, Arend Mulder, Frans Claas, Megan A. Cooper, Todd A. Fehniger

Research output: Contribution to journalArticle

Abstract

Natural killer (NK) cells are an emerging cellular immunotherapy for patients with acute myeloid leukemia (AML); however, the best approach to maximize NK cell antileukemia potential is unclear. Cytokine-induced memory-like NK cells differentiate after a brief preactivation with interleukin-12 (IL-12), IL-15, and IL-18 and exhibit enhanced responses to cytokine or activating receptor restimulation for weeks to months after preactivation. We hypothesized that memory-like NK cells exhibit enhanced antileukemia functionality. We demonstrated that human memory-like NK cells have enhanced interferon-g production and cytotoxicity against leukemia cell lines or primary human AML blasts in vitro. Usingmass cytometry, we found that memory-like NK cell functional responses were triggered against primary AML blasts, regardless of killer cell immunoglobulin-like receptor (KIR) to KIR-ligand interactions. In addition, multidimensional analyses identified distinct phenotypes of control and memory-like NK cells from the same individuals. Humanmemory-like NK cells xenografted into mice substantially reduced AML burden in vivo and improved overall survival. In the context of a first-in-human phase 1 clinical trial, adoptively transferred memory-like NK cells proliferated and expanded in AML patients and demonstrated robust responses against leukemia targets. Clinical responses were observed in five of nine evaluable patients, including four complete remissions. Thus, harnessing cytokine-induced memory-like NK cell responses represents a promising translational immunotherapy approach for patients with AML.

Original languageEnglish (US)
Article number357ra123
JournalScience Translational Medicine
Volume8
Issue number357
DOIs
StatePublished - Sep 21 2016
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Romee, R., Rosario, M., Berrien-Elliott, M. M., Wagner, J. A., Jewell, B. A., Schappe, T., Leong, J. W., Abdel-Latif, S., Schneider, S. E., Willey, S., Neal, C. C., Yu, L., Oh, S. T., Lee, Y. S., Mulder, A., Claas, F., Cooper, M. A., & Fehniger, T. A. (2016). Cytokine-induced memory-like natural killer cells exhibit enhanced responses against myeloid leukemia. Science Translational Medicine, 8(357), [357ra123]. https://doi.org/10.1126/scitranslmed.aaf2341