Cytokine-gene expression in measles-infected adult human glial cells

Toshio Yamabe, Gita Dhir, Elliot P. Cowan, Aizik L. Wolf, Gregory K Bergey, Allan Krumholz, Elizabeth Barry, Paul M. Hoffman, Suhayl Dhib-Jalbut

Research output: Contribution to journalArticle

Abstract

The expression of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF) α transcripts in cultured human glial cells was examined using reverse transcription followed by polymerase chain reaction (PCR) amplification and Southern blot quantitation. Microglial cultures derived from brain biopsy specimens from three different individuals expressed transcripts for the three cytokines under basal culture conditions. This expression was enhanced in response to measles virus (MV) infection (IL-1β, 2.2-8.8-fold; IL-6,2.5-8.4-fold; TNFα, 2.2-3.2-fold). Neither IL-1β nor TNFα transcripts were detectable in undissociated brain tissue from two individuals, suggesting that the basal expression of these cytokines in culture may have been induced by tissue dissociation or by the culture conditions. Oligodendrocytes did not express cytokine transcripts under basal culture conditions, and IL-1β and IL-6 but not TNFα transcripts could be induced by MV. Similarly, meningeal fibroblasts expressed IL-1β and IL-6 but not TNFα in response to MV-infection, suggesting that the production of TNFα is more cell type-restricted than either IL-1β or IL-6. The results indicate that adult human microglia can participate in the inflammatory response to MV infection in the CNS by producing cytokines that contribute to inflammation and demyelination. In addition, besides their role in myelination, oligodendrocytes can potentially influence immunoreactivity in the CNS by producing IL-1β and IL-6.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
JournalJournal of Neuroimmunology
Volume49
Issue number1-2
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Measles
Interleukin-1
Neuroglia
Interleukin-6
Measles virus
Cytokines
Tumor Necrosis Factor-alpha
Gene Expression
Virus Diseases
Oligodendroglia
Brain
Microglia
Demyelinating Diseases
Southern Blotting
Reverse Transcription
Fibroblasts
Inflammation
Biopsy
Polymerase Chain Reaction

Keywords

  • Interleukin-1β
  • Interleukin-6
  • Measles virus
  • Microglia
  • Tumor necrosis factor α

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Yamabe, T., Dhir, G., Cowan, E. P., Wolf, A. L., Bergey, G. K., Krumholz, A., ... Dhib-Jalbut, S. (1994). Cytokine-gene expression in measles-infected adult human glial cells. Journal of Neuroimmunology, 49(1-2), 171-179. https://doi.org/10.1016/0165-5728(94)90193-7

Cytokine-gene expression in measles-infected adult human glial cells. / Yamabe, Toshio; Dhir, Gita; Cowan, Elliot P.; Wolf, Aizik L.; Bergey, Gregory K; Krumholz, Allan; Barry, Elizabeth; Hoffman, Paul M.; Dhib-Jalbut, Suhayl.

In: Journal of Neuroimmunology, Vol. 49, No. 1-2, 1994, p. 171-179.

Research output: Contribution to journalArticle

Yamabe, T, Dhir, G, Cowan, EP, Wolf, AL, Bergey, GK, Krumholz, A, Barry, E, Hoffman, PM & Dhib-Jalbut, S 1994, 'Cytokine-gene expression in measles-infected adult human glial cells', Journal of Neuroimmunology, vol. 49, no. 1-2, pp. 171-179. https://doi.org/10.1016/0165-5728(94)90193-7
Yamabe, Toshio ; Dhir, Gita ; Cowan, Elliot P. ; Wolf, Aizik L. ; Bergey, Gregory K ; Krumholz, Allan ; Barry, Elizabeth ; Hoffman, Paul M. ; Dhib-Jalbut, Suhayl. / Cytokine-gene expression in measles-infected adult human glial cells. In: Journal of Neuroimmunology. 1994 ; Vol. 49, No. 1-2. pp. 171-179.
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