Cytokeratin expression in corneal dystrophies

Anamaria Baptista Coutinho, Denise De Freitas, João Pessoa De Souza Filho, Zélia Maria S. Corrêa, Alexandre Nakao Odashiro, Miguel N. Burnier

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To identify an immunohistochemical pattern of epithelial markers in granular, lattice and Avellino corneal dystrophies. Methods: Twenty-two corneal buttons, diagnosed as lattice (17), Avellino (4) and granular (1) underwent immunohistochemical studies of cytokeratins (CKs) on paraffin-embedded sections (group I). Monoclonal antibodies for pan-CK (AE1/AE3) and CKs 3/12, 5/6, 8, 18 and 19 were used. Twenty-two normal corneas were used as the control (group II). Results: Six lattice and 2 Avellino cases of group I stained positively with anti-CK 3/12 in corneal epithelium and areas of corneal stroma deposits. One of these cases of lattice was positive for anti-pan-CK (AE1/AE3) also in the epithelium and areas of corneal stroma deposits with a similar pattern. None of the controls (group II) revealed any staining in corneal stroma. All disease and control cases (groups I and II) revealed positive staining in corneal epithelium. Conclusion: AE1/AE3 and CK 3/12 anti-CK positive markers in the stromal deposits of lattice and Avellino dystrophies may suggest an epithelial genesis of the disease.

Original languageEnglish (US)
Pages (from-to)118-122
Number of pages5
JournalArquivos brasileiros de oftalmologia
Volume74
Issue number2
DOIs
StatePublished - Mar 2011

Keywords

  • Cornea/pathology
  • Corneal dystrophies
  • Hereditary
  • Immunohistochemistry
  • Keratins/physiology

ASJC Scopus subject areas

  • Ophthalmology

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