Cytogenetic studies of a human medullary thyroid carcinoma cell line

Kimio Tanaka, Stephen B Baylin, Barry D Nelkin, Joseph R. Testa

Research output: Contribution to journalArticle

Abstract

Detailed karyotypic analyses were performed on early and late passages of the TT cell line derived from malignant cells of a patient with the sporadic form of medullary thyroid carcinoma. Most of the cells examined were hypodiploid with a modal chromosome number of 43. The cells have a complex karyotype with ten rearranged chromosomes found in early passages and 12 rearranged chromosomes in late passages. The karyotypic pattern was relatively stable with continued in vitro culture, and consistent alterations involved chromosomes X, #1, #3, #5, #7, #8, #9, #10, #11, #12, and #14. Rearrangements of chromosome #11 are of particular interest, because the gene for calcitonin, the polypeptide hormone marker for medullary thyroid carcinoma resides on this chromosome. In early passages, one #11 contained an insertion within band q13, and this abnormality was retained in late passages. In late passage cells the other #11 homolog developed a deletion of part of the short arm, which involves the calcitonin gene region. We were unable to detect any deletion of chromosome band 20p12, previously reported by other investigators to occur in the germ line of patients with the hereditary form of medullary thyroid carcinoma.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalCancer Genetics and Cytogenetics
Volume25
Issue number1
DOIs
StatePublished - 1987

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Cytogenetics
Cell Line
Chromosomes
Calcitonin
Chromosomes, Human, Pair 12
Chromosome Deletion
Chromosomes, Human, Pair 11
Peptide Hormones
Chromosomes, Human, Pair 1
Karyotype
Germ Cells
Genes
Research Personnel
Medullary Thyroid cancer

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Cytogenetic studies of a human medullary thyroid carcinoma cell line. / Tanaka, Kimio; Baylin, Stephen B; Nelkin, Barry D; Testa, Joseph R.

In: Cancer Genetics and Cytogenetics, Vol. 25, No. 1, 1987, p. 27-35.

Research output: Contribution to journalArticle

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