Cytogenetic and ploidy analysis of prostatic adenocarcinoma.

E. J. Perlman, J. I. Epstein, P. P. Long, G. Pizov, C. A. Griffin

Research output: Contribution to journalArticlepeer-review

Abstract

The cytogenetic evaluation of prostatic adenocarcinoma has shown no consistent cytogenetic abnormalities. Despite manipulation of culture conditions, the majority of low-stage, untreated prostatic adenocarcinomas show a normal karyotype. We have performed cytogenetic analysis on eight primary prostate adenocarcinomas, using several control measures to increase the probability that any normal karyotype was derived from neoplastic cells rather than accompanying normal cells. Tumors were grown in media that encourages epithelial growth; DNA ploidy studies were performed before and after tissue culture; and immunohistochemical confirmation of the prostatic and epithelial nature of the cells was done following culture. Percentage of tumor on tissue sections adjacent to those submitted for culture was > 75% in all cases. Seven of eight cases were evaluable, and six cases showed no clonal abnormalities and were diploid. One tumor showed a population of tetraploid cells, without structural abnormalities. Three additional tumors showed evidence of tetraploidy by DNA analysis. One case showed nonclonal marker chromosomes and was aneuploid. This patient was pathologic Stage D. We conclude that the majority of prostatic adenocarcinomas at their inception may not show routinely detectable cytogenetic abnormalities. However, tetraploidy may play a role in the evolution of prostatic adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)348-352
Number of pages5
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume6
Issue number3
StatePublished - May 1993

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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