Cytochrome P450 1A1 and cytochrome P450 2E1 gene polymorphisms in Guangzhou Hans

J. Wang, Z. Liu, X. Lu, B. Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To determine the distribution of cytochrome P450 1A1 (CYP1A1) and cytochrome P450 2E1 (CYP2E1) gene polymorphisms in Guangzhou Hans. Methods: A total of 150 healthy Guangzhou Hans were studied with PCR-RFLP and ASA techniques. Current results were compared with the data on other ethnic groups. Results: In the 150 individuals tested, the frequencies of the ml(Msp I -) and m2(Msp I +) alleles of the Msp I polymorphic site in the 3' non-coding region of the CYP1A1 gene were found to be 62.33% and 37.67%, respectively. The observed frequencies of the m1m1, m1m2 and m2m2 genotypes were found to be 40%, 44.67% and 15.33%, respectively, which met Hardy-Weinberg equilibrium. The frequencies of the A and G alleles of the exon 7 A4889G polymorphic site in the CYP1A1 gene were found to be 79% and 21%, respectively. The observed frequencies of the AA, AG and GG genotypes were found to be 62.67%, 32.67% and 4.66%, respectively, which met Hardy-Weinberg equilibrium. A close linkage between the Msp I polymorphism and exon7 A4889G polymorphism was observed in Guangzhou Hans (X2= 62. 2358, P<0.005). The frequencies of the C1 (Rsa I +, Pst I -) and C2(Rsa I -, Pst I +) alleles of the Rsa I polymorphic site in the 5'-flanking region of the CYP2E1 gene were found to be 85.3% and 14.7%, respectively. The observed frequencies of the C1C1, C1C2 and C2C2 genotypes were found to be 67.3%, 29.3%, and 3.4%, respectively, which met Hardy-Weinberg equilibrium. Conclusion: The frequencies of the CYP1A1 and CYP2E1 gene polymorphisms in Guangzhou Hans were similar to those in Japanese population, but were significantly different from those in European populations.

Original languageEnglish (US)
Pages (from-to)259-261
Number of pages3
JournalChinese Journal of Medical Genetics
Volume17
Issue number4
StatePublished - Sep 14 2000

Keywords

  • Cytochrome P450
  • Polymerase chain reaction
  • Restriction fragment length polymorphism

ASJC Scopus subject areas

  • Genetics(clinical)

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