Cytochrome d induction in Escherichia coli growing under unfavorable conditions

A. V. Bogachev, R. A. Murtazina, V. P. Skulachev

Research output: Contribution to journalArticlepeer-review

Abstract

Growth of E. Coli in the presence of the protonophorous uncoupler pentachlorophenol is shown to strongly enhance levels of cytochrome d, a putative Na+-motive oxidase. This effect was found to be arrested by chloramphenicol and stimulated by high Na+ concentration in the growth medium. The induction of cytochrome d takes place in a mutant deficient in the F0F1 ATP-synthase but does not occur in mutants deficient in either of two different components of the Arc system. Similar relationships were revealed when pentachlorophenol was replaced by ferricyanide and phenazine methosulfate, agents oxidizing the respiratory chain. Induction of cytochrome d is also shown to occur in riboflavin-deficient mutants growing in the presence of such low riboflavin concentrations as to be insufficient to maintain a high respiration rate. It is suggested (i) that it is Δ-μH+ decrease rather than reduction of the respiratory chain that is the signal for the induction of cytochrome d, and (ii) the Arc system is involved in this type of metabolic regulation.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalFEBS Letters
Volume336
Issue number1
DOIs
StatePublished - Dec 20 1993

Keywords

  • Arc system
  • Cytochrome d
  • E.Coli
  • Na-motive oxidase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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