Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis

Wen Hsiang Lee; Ronald A. Morton; Jonathan I. Epstein; James D. Brooks; Pearl A. Campbell; G. Steven Bova; Wen Son Hsieh; William B. Isaacs; William G. Nelson

doi:10.1073/pnas.91.24.11733

Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis

Wen Hsiang Lee, Ronald A. Morton, Jonathan I. Epstein, James D. Brooks, Pearl A. Campbell, G. Steven Bova, Wen Son Hsieh, William B. Isaacs, William G. Nelson

School of Medicine

Research output: Contribution to journal › Article › peer-review

708 Scopus citations

Abstract

Hypermethylation of regulatory sequences at the locus of the π-class glutathione S-transferase gene GSTP1 was detected in 20 of 20 human prostatic carcinoma tissue specimens studied but not in normal tissues or prostatic tissues exhibiting benign hyperplasia. In addition, a striking decrease in GSTP1 expression was found to accompany human prostatic carcinogenesis. Immunohistochemical staining with anti-GSTP1 antibodies failed to detect the enzyme in 88 of 91 prostatic carcinomas analyzed. In vitro, GSTP1 expression was limited to human prostatic cancer cell lines containing GSTP1 alleles with hypomethylated promoter sequences; a human prostatic cancer cell line containing only hypermethylated GSTP1 promoter sequences did not express GSTP1 mRNA or polypeptides. Methylation of cytidine nucleotides in GSTP1 regulatory sequences constitutes the most common genomic alteration yet described for human prostate cancer.

Original language	English (US)
Pages (from-to)	11733-11737
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	24
DOIs	https://doi.org/10.1073/pnas.91.24.11733
State	Published - Nov 22 1994

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.91.24.11733

Cite this

Lee, W. H., Morton, R. A., Epstein, J. I., Brooks, J. D., Campbell, P. A., Bova, G. S., Hsieh, W. S., Isaacs, W. B., & Nelson, W. G. (1994). Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis. Proceedings of the National Academy of Sciences of the United States of America, 91(24), 11733-11737. https://doi.org/10.1073/pnas.91.24.11733

Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis. / Lee, Wen Hsiang; Morton, Ronald A.; Epstein, Jonathan I. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 24, 22.11.1994, p. 11733-11737.

Research output: Contribution to journal › Article › peer-review

Lee, WH, Morton, RA, Epstein, JI, Brooks, JD, Campbell, PA, Bova, GS, Hsieh, WS, Isaacs, WB & Nelson, WG 1994, 'Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 24, pp. 11733-11737. https://doi.org/10.1073/pnas.91.24.11733

@article{23f12d590fe5473794e1632dd124def7,

title = "Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis",

abstract = "Hypermethylation of regulatory sequences at the locus of the π-class glutathione S-transferase gene GSTP1 was detected in 20 of 20 human prostatic carcinoma tissue specimens studied but not in normal tissues or prostatic tissues exhibiting benign hyperplasia. In addition, a striking decrease in GSTP1 expression was found to accompany human prostatic carcinogenesis. Immunohistochemical staining with anti-GSTP1 antibodies failed to detect the enzyme in 88 of 91 prostatic carcinomas analyzed. In vitro, GSTP1 expression was limited to human prostatic cancer cell lines containing GSTP1 alleles with hypomethylated promoter sequences; a human prostatic cancer cell line containing only hypermethylated GSTP1 promoter sequences did not express GSTP1 mRNA or polypeptides. Methylation of cytidine nucleotides in GSTP1 regulatory sequences constitutes the most common genomic alteration yet described for human prostate cancer.",

author = "Lee, {Wen Hsiang} and Morton, {Ronald A.} and Epstein, {Jonathan I.} and Brooks, {James D.} and Campbell, {Pearl A.} and Bova, {G. Steven} and Hsieh, {Wen Son} and Isaacs, {William B.} and Nelson, {William G.}",

year = "1994",

month = nov,

day = "22",

doi = "10.1073/pnas.91.24.11733",

language = "English (US)",

volume = "91",

pages = "11733--11737",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "24",

}

TY - JOUR

T1 - Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis

AU - Lee, Wen Hsiang

AU - Morton, Ronald A.

AU - Epstein, Jonathan I.

AU - Brooks, James D.

AU - Campbell, Pearl A.

AU - Bova, G. Steven

AU - Hsieh, Wen Son

AU - Isaacs, William B.

AU - Nelson, William G.

PY - 1994/11/22

Y1 - 1994/11/22

N2 - Hypermethylation of regulatory sequences at the locus of the π-class glutathione S-transferase gene GSTP1 was detected in 20 of 20 human prostatic carcinoma tissue specimens studied but not in normal tissues or prostatic tissues exhibiting benign hyperplasia. In addition, a striking decrease in GSTP1 expression was found to accompany human prostatic carcinogenesis. Immunohistochemical staining with anti-GSTP1 antibodies failed to detect the enzyme in 88 of 91 prostatic carcinomas analyzed. In vitro, GSTP1 expression was limited to human prostatic cancer cell lines containing GSTP1 alleles with hypomethylated promoter sequences; a human prostatic cancer cell line containing only hypermethylated GSTP1 promoter sequences did not express GSTP1 mRNA or polypeptides. Methylation of cytidine nucleotides in GSTP1 regulatory sequences constitutes the most common genomic alteration yet described for human prostate cancer.

AB - Hypermethylation of regulatory sequences at the locus of the π-class glutathione S-transferase gene GSTP1 was detected in 20 of 20 human prostatic carcinoma tissue specimens studied but not in normal tissues or prostatic tissues exhibiting benign hyperplasia. In addition, a striking decrease in GSTP1 expression was found to accompany human prostatic carcinogenesis. Immunohistochemical staining with anti-GSTP1 antibodies failed to detect the enzyme in 88 of 91 prostatic carcinomas analyzed. In vitro, GSTP1 expression was limited to human prostatic cancer cell lines containing GSTP1 alleles with hypomethylated promoter sequences; a human prostatic cancer cell line containing only hypermethylated GSTP1 promoter sequences did not express GSTP1 mRNA or polypeptides. Methylation of cytidine nucleotides in GSTP1 regulatory sequences constitutes the most common genomic alteration yet described for human prostate cancer.

UR - http://www.scopus.com/inward/record.url?scp=0028152397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028152397&partnerID=8YFLogxK

U2 - 10.1073/pnas.91.24.11733

DO - 10.1073/pnas.91.24.11733

M3 - Article

C2 - 7972132

AN - SCOPUS:0028152397

SN - 0027-8424

VL - 91

SP - 11733

EP - 11737

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

Cytidine methylation of regulatory sequences near the π-class glutathione S-transferase gene accompanies human prostatic carcinogenesis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this