TY - JOUR
T1 - Cystic fibrosis transmembrane conductance regulator expression in human hypothalamus
AU - Mulberg, Andrew E.
AU - Weyler, Richard T.
AU - Altschuler, Steven M.
AU - Hyde, Thomas M.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1998/1/5
Y1 - 1998/1/5
N2 - WE have previously characterized the expression of the cystic fibrosis transmembrane conductance regulator protein (CFTR) gene, mRNA and protein in rat brain with reverse transcriptase (RT)-PCR amplification, in situ hybridization and immunocytochemistry. We now report that the CFTR mRNA is expressed in the human anterior hypothalamus, an area involved in regulation of appetite, resting energy expenditure and sexual differentiation. Expression of CFTR in neurons localized to this region may elucidate the pathogenesis of other nonpulmonary manifestations of cystic fibrosis which commonly are observed in children with CF, including congenital absence of the vas deferens. Neuron-specific expression of CFTR in brain may be involved in the regulation of homeostatic functions including reproductive function and fertility through effects on neurosecretion, i.e. GnRH release. Dysregulation of normal neuropeptide vesicle trafficking by mutant CFTR in brain my lead to alteration in physiological function.
AB - WE have previously characterized the expression of the cystic fibrosis transmembrane conductance regulator protein (CFTR) gene, mRNA and protein in rat brain with reverse transcriptase (RT)-PCR amplification, in situ hybridization and immunocytochemistry. We now report that the CFTR mRNA is expressed in the human anterior hypothalamus, an area involved in regulation of appetite, resting energy expenditure and sexual differentiation. Expression of CFTR in neurons localized to this region may elucidate the pathogenesis of other nonpulmonary manifestations of cystic fibrosis which commonly are observed in children with CF, including congenital absence of the vas deferens. Neuron-specific expression of CFTR in brain may be involved in the regulation of homeostatic functions including reproductive function and fertility through effects on neurosecretion, i.e. GnRH release. Dysregulation of normal neuropeptide vesicle trafficking by mutant CFTR in brain my lead to alteration in physiological function.
KW - Cystic fibrosis transmembrane conductance regulator (CFTR)
KW - Fertility
KW - Gonadotropin-releasing hormone (GnRH)
KW - Human anterior hypothalamus
KW - Non-isotopic in situ hybridization (NISH)
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U2 - 10.1097/00001756-199801050-00028
DO - 10.1097/00001756-199801050-00028
M3 - Article
C2 - 9592064
AN - SCOPUS:0032484636
SN - 0959-4965
VL - 9
SP - 141
EP - 144
JO - Neuroreport
JF - Neuroreport
IS - 1
ER -