Cysteine proteinase-1 and cut protein isoform control dendritic innervation of two distinct sensory fields by a single neuron

Gray R. Lyons, Ryan O. Andersen, Khadar Abdi, Won Seok Song, Chay T. Kuo

Research output: Contribution to journalArticle

Abstract

Dendrites often exhibit structural changes in response to local inputs. Although mechanisms that pattern and maintain dendritic arbors are becoming clearer, processes regulating regrowth, during context-dependent plasticity or after injury, remain poorly understood. We found that a class of Drosophila sensory neurons, through complete pruning and regeneration, can elaborate two distinct dendritic trees, innervating independent sensory fields. An expression screen identified Cysteine proteinase-1 (Cp1) as a critical regulator of this process. Unlike known ecdysone effectors, Cp1-mutant ddaC neurons pruned larval dendrites normally but failed to regrow adult dendrites. Cp1 expression was upregulated/concentrated in the nucleus during metamorphosis, controlling production of a truncated Cut homeodomain transcription factor. This truncated Cut, but not the full-length protein, allowed Cp1-mutant ddaC neurons to regenerate higher-order adult dendrites. These results identify a molecular pathway needed for dendrite regrowth after pruning, which allows the same neuron to innervate distinct sensory fields.

Original languageEnglish (US)
Pages (from-to)783-791
Number of pages9
JournalCell Reports
Volume6
Issue number5
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

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Cysteine Proteases
Dendrites
Neurons
Protein Isoforms
Ecdysone
Plasticity
Sensory Receptor Cells
Transcription Factors
Drosophila
Regeneration
Wounds and Injuries
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cysteine proteinase-1 and cut protein isoform control dendritic innervation of two distinct sensory fields by a single neuron. / Lyons, Gray R.; Andersen, Ryan O.; Abdi, Khadar; Song, Won Seok; Kuo, Chay T.

In: Cell Reports, Vol. 6, No. 5, 01.01.2014, p. 783-791.

Research output: Contribution to journalArticle

Lyons, Gray R. ; Andersen, Ryan O. ; Abdi, Khadar ; Song, Won Seok ; Kuo, Chay T. / Cysteine proteinase-1 and cut protein isoform control dendritic innervation of two distinct sensory fields by a single neuron. In: Cell Reports. 2014 ; Vol. 6, No. 5. pp. 783-791.
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