Cyclosporine a impairs wound healing in rats

Rhonda Fishel, Adrian Barbul, Hannah L. Wasserkrug, Lynne T. Penberthy, Giuseppe Rettura, Gershon Efron

Research output: Contribution to journalArticlepeer-review

Abstract

Cellular immune responses may play an important role in the early inflammatory and cellular phases of wound healing. Cyclosporine A (CSA), a new immunosuppressive agent, impairs cellular immunity and T-cell-dependent humoral immunity. Therefore, the effect of CSA-induced immunosuppression in a rat wound-healing model was studied. Sprague-Dawley rats underwent a standardized skin incision and subcutaneous implantation of sterile polyvinyl alcohol sponges. CSA was dissolved in olive oil and given by gavage to one group of animals at a total dose of 125 mg/kg/10 days. The control group received an equivalent volume of olive oil. Ten-day-old wounds were weaker in CSA-treated animals, both in the fresh state (282 ± 19 g vs 380 ± 27 g, P < 0.01), and after formalin fixation (1111 ± 74 g vs 1419 ± 57 g, P < 0.01). In addition, CSA-treated rats accumulated significantly less hydroxyproline in the wound sponge granuloma, an index of reparative collagen deposition. The impairment in wound healing occurred without differences in body weight gain or organ weights. There was a profound immunosuppression in the animals receiving CSA as determined by thymic lymphocyte blastogenesis in response to Con A and PHA. These findings suggest that immunosuppression in otherwise healthy animals impairs wound healing.

Original languageEnglish (US)
Pages (from-to)572-575
Number of pages4
JournalJournal of Surgical Research
Volume34
Issue number6
DOIs
StatePublished - Jun 1983

ASJC Scopus subject areas

  • Surgery

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