TY - JOUR
T1 - Cyclosporin-a sensitive induction of NF-AT in murine B cells
AU - Venkataraman, Lakshmi
AU - Francis, Delicia A.
AU - Wang, Zihua
AU - Liu, Jialing
AU - Rothstein, Thomas L.
AU - Sen, Ranjan
PY - 1994
Y1 - 1994
N2 - Primary B cells are induced to proliferate by crosslinking surface immunoglobulin or by its pharmacological equivalent, phorbol ester and calcium ionophore. However, nuclear responses that have been studied in activated B cells are typically inducible with phorbol esters alone. We show that a factor, indistinguishable from the nuclear factor of activated T cells (NF-AT), is induced in B cells in response to anti-immunoglobulin signals or the combined action of phorbol ester and ionomycin, but not in response to either reagent alone. The signals necessary for NF-AT induction in B cells, therefore, closely parallel those required to induce B cell proliferation. Transfection analysis shows that B cell NF-AT is a transcriptional activator. Furthermore, NF-AT induction in spienic cells is suppressed by cyclosporin A, suggesting a mechanism by which immunosuppressive agents act on the B cell compartment. We propose that NF-AT should be considered more generally as a nuclear factor of activated lymphoid cells.
AB - Primary B cells are induced to proliferate by crosslinking surface immunoglobulin or by its pharmacological equivalent, phorbol ester and calcium ionophore. However, nuclear responses that have been studied in activated B cells are typically inducible with phorbol esters alone. We show that a factor, indistinguishable from the nuclear factor of activated T cells (NF-AT), is induced in B cells in response to anti-immunoglobulin signals or the combined action of phorbol ester and ionomycin, but not in response to either reagent alone. The signals necessary for NF-AT induction in B cells, therefore, closely parallel those required to induce B cell proliferation. Transfection analysis shows that B cell NF-AT is a transcriptional activator. Furthermore, NF-AT induction in spienic cells is suppressed by cyclosporin A, suggesting a mechanism by which immunosuppressive agents act on the B cell compartment. We propose that NF-AT should be considered more generally as a nuclear factor of activated lymphoid cells.
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U2 - 10.1016/1074-7613(94)90097-3
DO - 10.1016/1074-7613(94)90097-3
M3 - Article
C2 - 7889407
AN - SCOPUS:0028445321
VL - 1
SP - 189
EP - 196
JO - Immunity
JF - Immunity
SN - 1074-7613
IS - 3
ER -