Cyclosporin-a sensitive induction of NF-AT in murine B cells

Lakshmi Venkataraman, Delicia A. Francis, Zihua Wang, Jialing Liu, Thomas L. Rothstein, Ranjan Sen

Research output: Contribution to journalArticle

Abstract

Primary B cells are induced to proliferate by crosslinking surface immunoglobulin or by its pharmacological equivalent, phorbol ester and calcium ionophore. However, nuclear responses that have been studied in activated B cells are typically inducible with phorbol esters alone. We show that a factor, indistinguishable from the nuclear factor of activated T cells (NF-AT), is induced in B cells in response to anti-immunoglobulin signals or the combined action of phorbol ester and ionomycin, but not in response to either reagent alone. The signals necessary for NF-AT induction in B cells, therefore, closely parallel those required to induce B cell proliferation. Transfection analysis shows that B cell NF-AT is a transcriptional activator. Furthermore, NF-AT induction in spienic cells is suppressed by cyclosporin A, suggesting a mechanism by which immunosuppressive agents act on the B cell compartment. We propose that NF-AT should be considered more generally as a nuclear factor of activated lymphoid cells.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalImmunity
Volume1
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases

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  • Cite this

    Venkataraman, L., Francis, D. A., Wang, Z., Liu, J., Rothstein, T. L., & Sen, R. (1994). Cyclosporin-a sensitive induction of NF-AT in murine B cells. Immunity, 1(3), 189-196. https://doi.org/10.1016/1074-7613(94)90097-3