Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells

H. J. Broxterman, H. M. Pinedo, G. J. Schuurhuis, J. Lankelma

Research output: Contribution to journalArticle

Abstract

Cyclosporin A (Sandimmune®) rapidly induced an increase in daunorubicin accumulation in multidrug-resistant human ovarian carcinoma cells (2780AD) and was more potent than verapamil. Steady-state 3H-cyclosporin A accumulation at 37°C in 2780AD cells was 60-70% of that in the sensitive A2780 cells. A rapid increase of ATP consumption and lactate production was induced in 2780AD cells by verapamil, but not by cyclosporin A. These results suggest that the interactions of cyclosporin A and verapamil with P-glycoprotein, which leads to inhibition of drug transport, have a different mechanistic basis.

Original languageEnglish (US)
Pages (from-to)85-88
Number of pages4
JournalBritish Journal of Cancer
Volume62
Issue number1
StatePublished - 1990
Externally publishedYes

Fingerprint

Verapamil
Energy Metabolism
Cyclosporine
Neoplasms
Daunorubicin
P-Glycoprotein
Lactic Acid
Adenosine Triphosphate
Carcinoma
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Broxterman, H. J., Pinedo, H. M., Schuurhuis, G. J., & Lankelma, J. (1990). Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells. British Journal of Cancer, 62(1), 85-88.

Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells. / Broxterman, H. J.; Pinedo, H. M.; Schuurhuis, G. J.; Lankelma, J.

In: British Journal of Cancer, Vol. 62, No. 1, 1990, p. 85-88.

Research output: Contribution to journalArticle

Broxterman, HJ, Pinedo, HM, Schuurhuis, GJ & Lankelma, J 1990, 'Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells', British Journal of Cancer, vol. 62, no. 1, pp. 85-88.
Broxterman, H. J. ; Pinedo, H. M. ; Schuurhuis, G. J. ; Lankelma, J. / Cyclosporin A and verapamil have different effects on energy metabolism in multidrug-resistant tumour cells. In: British Journal of Cancer. 1990 ; Vol. 62, No. 1. pp. 85-88.
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