Cyclosporin A and the thymus: Immunopathology

W. E. Beschorner, J. D. Namnoum, A. D. Hess, C. A. Shinn, G. W. Santos

Research output: Contribution to journalArticle

Abstract

Cyclosporin A (CsA) is known to diminish the size of the thymus, especially the thymic medulla. The significance of these changes is not presently understood. This study reveals several immunopathologic changes induced in the thymic medulla by CsA (15 mg/kg/day). The weight and relative size of the thymus dramatically and rapidly involutes, with marked changes observed in 1 week. The medullary thymocytes show segregation of rat T-cell phenotypes as seen in control rats, but the number of such cells is markedly reduced in accordance with the medullary remnant. This is consistent with a maturational arrest of thymocytes. The medullary epithelium was assessed directly by irradiating the control or CsA-treated rats 2 days prior to sacrifice. The epithelium of Hassall's corpuscles was essentially absent in CsA-treated rats but prominent in control rats. The cortical epithelial cells were preserved. Stains for Ia antigen with the anti-OX4 antibody show little change in expression by cortical epithelium, but a marked reduction in the Ia+ medullary cells in the thymocyte purged rats. All of these changes were reversible in the normal rat after cessation of CsA, with near normal recovery in 3 weeks. No morphologic or immunopathologic changes were noted in the cortical thymocytes. These cells did, however, acquire CsA receptors, as detected by the binding of fluorescent dansylated CsA.

Original languageEnglish (US)
Pages (from-to)487-496
Number of pages10
JournalAmerican Journal of Pathology
Volume126
Issue number3
StatePublished - 1987

Fingerprint

Thymus Gland
Cyclosporine
Thymocytes
Epithelium
Histocompatibility Antigens Class II
Anti-Idiotypic Antibodies
Coloring Agents
Cell Count
Epithelial Cells
T-Lymphocytes
Phenotype
Weights and Measures

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Beschorner, W. E., Namnoum, J. D., Hess, A. D., Shinn, C. A., & Santos, G. W. (1987). Cyclosporin A and the thymus: Immunopathology. American Journal of Pathology, 126(3), 487-496.

Cyclosporin A and the thymus : Immunopathology. / Beschorner, W. E.; Namnoum, J. D.; Hess, A. D.; Shinn, C. A.; Santos, G. W.

In: American Journal of Pathology, Vol. 126, No. 3, 1987, p. 487-496.

Research output: Contribution to journalArticle

Beschorner, WE, Namnoum, JD, Hess, AD, Shinn, CA & Santos, GW 1987, 'Cyclosporin A and the thymus: Immunopathology', American Journal of Pathology, vol. 126, no. 3, pp. 487-496.
Beschorner WE, Namnoum JD, Hess AD, Shinn CA, Santos GW. Cyclosporin A and the thymus: Immunopathology. American Journal of Pathology. 1987;126(3):487-496.
Beschorner, W. E. ; Namnoum, J. D. ; Hess, A. D. ; Shinn, C. A. ; Santos, G. W. / Cyclosporin A and the thymus : Immunopathology. In: American Journal of Pathology. 1987 ; Vol. 126, No. 3. pp. 487-496.
@article{5180d36236894a76a7264b7cd75a5d7e,
title = "Cyclosporin A and the thymus: Immunopathology",
abstract = "Cyclosporin A (CsA) is known to diminish the size of the thymus, especially the thymic medulla. The significance of these changes is not presently understood. This study reveals several immunopathologic changes induced in the thymic medulla by CsA (15 mg/kg/day). The weight and relative size of the thymus dramatically and rapidly involutes, with marked changes observed in 1 week. The medullary thymocytes show segregation of rat T-cell phenotypes as seen in control rats, but the number of such cells is markedly reduced in accordance with the medullary remnant. This is consistent with a maturational arrest of thymocytes. The medullary epithelium was assessed directly by irradiating the control or CsA-treated rats 2 days prior to sacrifice. The epithelium of Hassall's corpuscles was essentially absent in CsA-treated rats but prominent in control rats. The cortical epithelial cells were preserved. Stains for Ia antigen with the anti-OX4 antibody show little change in expression by cortical epithelium, but a marked reduction in the Ia+ medullary cells in the thymocyte purged rats. All of these changes were reversible in the normal rat after cessation of CsA, with near normal recovery in 3 weeks. No morphologic or immunopathologic changes were noted in the cortical thymocytes. These cells did, however, acquire CsA receptors, as detected by the binding of fluorescent dansylated CsA.",
author = "Beschorner, {W. E.} and Namnoum, {J. D.} and Hess, {A. D.} and Shinn, {C. A.} and Santos, {G. W.}",
year = "1987",
language = "English (US)",
volume = "126",
pages = "487--496",
journal = "American Journal of Pathology",
issn = "0002-9440",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Cyclosporin A and the thymus

T2 - Immunopathology

AU - Beschorner, W. E.

AU - Namnoum, J. D.

AU - Hess, A. D.

AU - Shinn, C. A.

AU - Santos, G. W.

PY - 1987

Y1 - 1987

N2 - Cyclosporin A (CsA) is known to diminish the size of the thymus, especially the thymic medulla. The significance of these changes is not presently understood. This study reveals several immunopathologic changes induced in the thymic medulla by CsA (15 mg/kg/day). The weight and relative size of the thymus dramatically and rapidly involutes, with marked changes observed in 1 week. The medullary thymocytes show segregation of rat T-cell phenotypes as seen in control rats, but the number of such cells is markedly reduced in accordance with the medullary remnant. This is consistent with a maturational arrest of thymocytes. The medullary epithelium was assessed directly by irradiating the control or CsA-treated rats 2 days prior to sacrifice. The epithelium of Hassall's corpuscles was essentially absent in CsA-treated rats but prominent in control rats. The cortical epithelial cells were preserved. Stains for Ia antigen with the anti-OX4 antibody show little change in expression by cortical epithelium, but a marked reduction in the Ia+ medullary cells in the thymocyte purged rats. All of these changes were reversible in the normal rat after cessation of CsA, with near normal recovery in 3 weeks. No morphologic or immunopathologic changes were noted in the cortical thymocytes. These cells did, however, acquire CsA receptors, as detected by the binding of fluorescent dansylated CsA.

AB - Cyclosporin A (CsA) is known to diminish the size of the thymus, especially the thymic medulla. The significance of these changes is not presently understood. This study reveals several immunopathologic changes induced in the thymic medulla by CsA (15 mg/kg/day). The weight and relative size of the thymus dramatically and rapidly involutes, with marked changes observed in 1 week. The medullary thymocytes show segregation of rat T-cell phenotypes as seen in control rats, but the number of such cells is markedly reduced in accordance with the medullary remnant. This is consistent with a maturational arrest of thymocytes. The medullary epithelium was assessed directly by irradiating the control or CsA-treated rats 2 days prior to sacrifice. The epithelium of Hassall's corpuscles was essentially absent in CsA-treated rats but prominent in control rats. The cortical epithelial cells were preserved. Stains for Ia antigen with the anti-OX4 antibody show little change in expression by cortical epithelium, but a marked reduction in the Ia+ medullary cells in the thymocyte purged rats. All of these changes were reversible in the normal rat after cessation of CsA, with near normal recovery in 3 weeks. No morphologic or immunopathologic changes were noted in the cortical thymocytes. These cells did, however, acquire CsA receptors, as detected by the binding of fluorescent dansylated CsA.

UR - http://www.scopus.com/inward/record.url?scp=0023094525&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023094525&partnerID=8YFLogxK

M3 - Article

C2 - 3493702

AN - SCOPUS:0023094525

VL - 126

SP - 487

EP - 496

JO - American Journal of Pathology

JF - American Journal of Pathology

SN - 0002-9440

IS - 3

ER -