Cyclooxygenase-2 is expressed in bladder during fetal development and stimulated by outlet obstruction

John M. Park, Tianxin Yang, Lois J Arend, Ann M. Smart, Jurgen B. Schnermann, Josephine P. Briggs

Research output: Contribution to journalArticle

Abstract

Studies were undertaken to assess expression of inducible cyclooxygenase (COX)-2 in bladder during fetal development and COX-1 and COX-2 expression after outlet obstruction. Bladder tissue or bladder progenitor tissue was harvested from CD-1 murine embryos at embryonic days 11.5 (E11.5), E14.5, E17.5, E20.5 (newborn), and from adult. Bladder obstruction was created in adult female mice by ligating the urethra, and bladders were harvested after 3-24 h of obstruction. Gene expression was assessed by semiquantitative reverse transcription-polymerase chain reaction and Western blotting. COX-2 was highly expressed at the early stages of bladder development and declined progressively throughout gestation. In adult bladder, both COX-1 and COX-2 were detectable at low levels under basal conditions. An ~30-fold increase in COX-2 mRNA was seen after 24 h of obstruction. In contrast, COX-1 did not change with obstruction. COX-2 mRNA levels peaked at 6 h of obstruction. In regional bladder-distention models, COX-2 induction was confined to the area of distention. Bladder outlet obstruction stimulates COX-2 expression dramatically, reactivating a gene that is highly expressed during fetal development.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume273
Issue number4 42-4
StatePublished - Oct 1997

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Cyclooxygenase 2
Fetal Development
Urinary Bladder
Cyclooxygenase 1
Urinary Bladder Neck Obstruction
Messenger RNA
Urethra
Reverse Transcription
Embryonic Structures
Western Blotting
Gene Expression
Pregnancy
Polymerase Chain Reaction
Genes

Keywords

  • Distention
  • Embryogenesis
  • Genitourinary system
  • Prostaglandin
  • Stretch

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Cyclooxygenase-2 is expressed in bladder during fetal development and stimulated by outlet obstruction. / Park, John M.; Yang, Tianxin; Arend, Lois J; Smart, Ann M.; Schnermann, Jurgen B.; Briggs, Josephine P.

In: American Journal of Physiology - Renal Physiology, Vol. 273, No. 4 42-4, 10.1997.

Research output: Contribution to journalArticle

Park, John M. ; Yang, Tianxin ; Arend, Lois J ; Smart, Ann M. ; Schnermann, Jurgen B. ; Briggs, Josephine P. / Cyclooxygenase-2 is expressed in bladder during fetal development and stimulated by outlet obstruction. In: American Journal of Physiology - Renal Physiology. 1997 ; Vol. 273, No. 4 42-4.
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