Cyclooxygenase-2 expression during carcinogenesis in the human stomach

Bastiaan P. Van Rees, Kirsi Saukkonen, Ari Ristimki, Wojciech Polkowski, Guido N J Tytgat, Paul Drillenburg, G. Johan A Offerhaus

Research output: Contribution to journalArticle

Abstract

The prolonged use of non-steroidal anti-inflammatory drugs (NSAIDs) has been associated with a reduced risk of gastric cancer. The best-known target of these drugs is cyclooxygenase (COX); the COX-2 isoform is frequently up-regulated in gastric adenocarcinomas. Using the post-gastrectomy stomach as a model, the expression of COX-2 mRNA and protein has been investigated during tumour progression in the human stomach. COX-2 expression was comparable in gastric stump carcinomas and conventional gastric carcinomas and localized primarily to the cytoplasm of the neoplastic cells. COX-2 mRNA was elevated in biopsies containing intestinal metaplasia, as determined by reverse transcriptase polymerase chain reaction (RT-PCR). COX-2 immunopositivity became more frequent during progression from reactive epithelium to high-grade dysplasia, both in the epithelial and in the stromal cell compartment. Co-localization of COX-2-positive stromal cells was seen with CD68, α-smooth muscle actin (α-SMA), vimentin, and HLA-DR, but an as yet unidentified subpopulation of stromal cells remained. Co-localization with the macrophage marker CD68 was only observed in a minority of COX-2-positive cells. These data show that COX-2 expression is a relatively early event during carcinogenesis in the stomach. COX-2 expression increases during tumour progression in the stomach, suggesting a role for COX-2 expression in gastric tumourigenesis.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
JournalJournal of Pathology
Volume196
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Stomach
Carcinogenesis
Stromal Cells
Gastric Stump
Carcinoma
Messenger RNA
Metaplasia
HLA-DR Antigens
Vimentin
Gastrectomy
Prostaglandin-Endoperoxide Synthases
Reverse Transcriptase Polymerase Chain Reaction
Pharmaceutical Preparations
Stomach Neoplasms
Smooth Muscle
Actins
Neoplasms
Protein Isoforms
Cytoplasm

Keywords

  • Cancer
  • Carcinogenesis
  • Cyclooxygenase
  • Stomach
  • Tumour progression

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Van Rees, B. P., Saukkonen, K., Ristimki, A., Polkowski, W., Tytgat, G. N. J., Drillenburg, P., & Johan A Offerhaus, G. (2002). Cyclooxygenase-2 expression during carcinogenesis in the human stomach. Journal of Pathology, 196(2), 171-179. https://doi.org/10.1002/path.1033

Cyclooxygenase-2 expression during carcinogenesis in the human stomach. / Van Rees, Bastiaan P.; Saukkonen, Kirsi; Ristimki, Ari; Polkowski, Wojciech; Tytgat, Guido N J; Drillenburg, Paul; Johan A Offerhaus, G.

In: Journal of Pathology, Vol. 196, No. 2, 2002, p. 171-179.

Research output: Contribution to journalArticle

Van Rees, BP, Saukkonen, K, Ristimki, A, Polkowski, W, Tytgat, GNJ, Drillenburg, P & Johan A Offerhaus, G 2002, 'Cyclooxygenase-2 expression during carcinogenesis in the human stomach', Journal of Pathology, vol. 196, no. 2, pp. 171-179. https://doi.org/10.1002/path.1033
Van Rees BP, Saukkonen K, Ristimki A, Polkowski W, Tytgat GNJ, Drillenburg P et al. Cyclooxygenase-2 expression during carcinogenesis in the human stomach. Journal of Pathology. 2002;196(2):171-179. https://doi.org/10.1002/path.1033
Van Rees, Bastiaan P. ; Saukkonen, Kirsi ; Ristimki, Ari ; Polkowski, Wojciech ; Tytgat, Guido N J ; Drillenburg, Paul ; Johan A Offerhaus, G. / Cyclooxygenase-2 expression during carcinogenesis in the human stomach. In: Journal of Pathology. 2002 ; Vol. 196, No. 2. pp. 171-179.
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