Cyclodextrin Modulated Type I Collagen Self-Assembly to Engineer Biomimetic Cornea Implants

Shoumyo Majumdar, Xiaokun Wang, Sven D. Sommerfeld, Jemin Jeremy Chae, Evangelia Nefeli Athanasopoulou, Lucas S. Shores, Xiaodong Duan, Mario L Amzel, Francesco Stellacci, Oliver D Schein, Qiongyu Guo, Anirudha Singh, Jennifer Hartt Elisseeff

Research output: Contribution to journalArticle

Abstract

Collagen-rich tissues in the cornea exhibit unique and highly organized extracellular matrix ultrastructures, which contribute to its high load-bearing capacity and light transmittance. Corneal collagen fibrils are controlled during development by small leucine-rich proteoglycans (SLRPs) that regulate the fibril diameter and spacing in order to achieve the unique optical transparency. Cyclodextrins (CDs) of varying size and chemical functionality for their ability to regulate collagen assembly during vitrification process are screened in order to create biosynthetic materials that mimic the native cornea structure. Addition of βCD to collagen vitrigels produces materials with aligned fibers and lamellae similar to native cornea, resulting in mechanically robust and transparent materials. Biochemistry analysis revealed that CD interacts with hydrophobic amino acids in collagen to influence assembly and fibril organization. To translate the self-assembled collagen materials for cornea reconstruction, custom molds for gelation and vitrification are engineered to create βCD/Col implants with curvature matching that of the cornea. Acellular βCD/Col materials are implanted in a rabbit partial keratoplasty model with interrupted sutures. The implants demonstrate tissue integration and support re-epithelialization. Therefore, the addition of CD molecules regulates collagen self-assembly and provides a simple process to engineer corneal mimetic substitutes with advanced structural and functional properties.

Original languageEnglish (US)
JournalAdvanced Functional Materials
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

cornea
Cyclodextrins
biomimetics
Biomimetics
collagens
Collagen Type I
Collagen
Self assembly
engineers
self assembly
Engineers
vitrification
Vitrification
assembly
Tissue
leucine
biochemistry
Biochemistry
transparence
rabbits

Keywords

  • collagen
  • cornea
  • cyclodextrins
  • fibril alignment
  • self-assembly

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Condensed Matter Physics
  • Electrochemistry

Cite this

Cyclodextrin Modulated Type I Collagen Self-Assembly to Engineer Biomimetic Cornea Implants. / Majumdar, Shoumyo; Wang, Xiaokun; Sommerfeld, Sven D.; Chae, Jemin Jeremy; Athanasopoulou, Evangelia Nefeli; Shores, Lucas S.; Duan, Xiaodong; Amzel, Mario L; Stellacci, Francesco; Schein, Oliver D; Guo, Qiongyu; Singh, Anirudha; Elisseeff, Jennifer Hartt.

In: Advanced Functional Materials, 01.01.2018.

Research output: Contribution to journalArticle

Majumdar, Shoumyo ; Wang, Xiaokun ; Sommerfeld, Sven D. ; Chae, Jemin Jeremy ; Athanasopoulou, Evangelia Nefeli ; Shores, Lucas S. ; Duan, Xiaodong ; Amzel, Mario L ; Stellacci, Francesco ; Schein, Oliver D ; Guo, Qiongyu ; Singh, Anirudha ; Elisseeff, Jennifer Hartt. / Cyclodextrin Modulated Type I Collagen Self-Assembly to Engineer Biomimetic Cornea Implants. In: Advanced Functional Materials. 2018.
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