Cyclobuta-dithymidine induction by solar-simulating UV radiation in human skin: II. Individual responses

Magnus Bruze, Edward A. Emmet, Jessica Creasey, Paul T. Strickland

Research output: Contribution to journalArticle

Abstract

The purpose of this study was to examine cyclobuta-dithymidine (TOT) photoproduct induction and persistence in human skin exposed in situ to simulated solar UV radiation. Small areas of untanned skin in nineteen individuals were exposed using a solar UV apparatus that simulates both the spectrum and intensity of the UV portion of summer midday sunlight at 39°N latitude. The equivalent of approximately 60 min of sunlight exposure (72KJ/m2) was administered, and TOT photoproducts were quantitated by enzyme-linked immunosorbent assay (ELISA) of DNA extracted from skin punch biopsies. Net yields of TOT photoproducts were determined in 10 individuals, with the majority (7 of 10) between 0.02 and 0.12 TOT per kilobase (Kb). The three remaining individuals had higher levels of photoproducts but were not unusually sensitive to solar UV as determined by minimal erythema dose (MED). Percentage loss of TOT photoproducts 4 h after exposure was determined in nine individuals to be 80.2 ±14.0%.

Original languageEnglish (US)
Pages (from-to)341-344
Number of pages4
JournalJournal of Investigative Dermatology
Volume93
Issue number3
DOIs
StatePublished - Sep 1989

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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