Abstract
Accumulation of the V(D)J recombinase protein RAG-2 is restricted to G0/G1 cells by phosphorylation-mediated degradation at the G1-S boundary. Here cyclin A/CDK2 is shown to oppose RAG-2 accumulation; conversely, RAG-2 is induced by p27(Kip1) and related CDK inhibitors. Coinduction of RAG-2 and G1 delay by p27(Kip1) is accompanied by strong stimulation of V(D)J recombination. Unexpectedly, induction of RAG-2 accumulation in the absence of G1 delay has no effect on recombination frequency, p27(Kip1) may stimulate V(D)J recombination by coordinating accumulation of RAG-2 with prolongation of G1, when nonhomologous end joining is preferentially active. Consistent with this, enforced expression of RAG-2 throughout cell cycle is associated with accumulation of aberrant recombination products reminiscent of those formed in the absence of nonhomologous end joining.
Original language | English (US) |
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Pages (from-to) | 771-781 |
Number of pages | 11 |
Journal | Immunity |
Volume | 11 |
Issue number | 6 |
DOIs | |
State | Published - Dec 1999 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Infectious Diseases