Cyclin A associates with the fusome during germline cyst formation in the Drosophila ovary

Mary A. Lilly, Margaret De Cuevas, Allan C. Spradling

Research output: Contribution to journalArticle

Abstract

Regulated changes in the cell cycle underlie many aspects of growth and differentiation. Prior to meiosis, germ cell cycles in many organisms become accelerated, synchronized, and modified to lack cytokinesis. These changes cause cysts of interconnected germ cells to form that typically contain 2(n) cells. In Drosophila, developing germ cells during this period contain a distinctive organelle, the fusome, that is required for normal cyst formation. We find that the cell cycle regulator Cyclin A transiently associates with the fusome during the cystocyte cell cycles, suggesting that fusome-associated Cyclin A drives the interconnected cells within each cyst synchronously into mitosis. In the presence of a normal fusome, overexpression of Cyclin A forces cysts through an extra round of cell division to produce cysts with 32 germline cells. Female sterile mutations in UbcD1, encoding an E2 ubiquitin-conjugating enzyme, have a similar effect. Our observations suggest that programmed changes in the expression and cytoplasmic localization of key cell cycle regulatory proteins control germline cyst production. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalDevelopmental biology
Volume218
Issue number1
DOIs
StatePublished - Feb 1 2000
Externally publishedYes

Keywords

  • Cell cycle
  • Cyclin A
  • Drosophila
  • Fusome
  • Oogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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