Cyclic strain stimulates proliferative capacity, α2 and α5 integrin, gene marker expression by human articular chondrocytes propagated on flexible silicone membranes

Kian Lahiji, Anna Polotsky, David S. Hungerford, Carmelita G. Frondoza

Research output: Contribution to journalArticle

Abstract

Chondrocytes comprise less than 10% of cartilage tissue but are responsible for sensing and responding to mechanical stimuli imposed on the joint. However, the effect of mechanical signals at the cellular level is not yet fully defined. The purpose of this study was to test the hypothesis that mechanical stimulation in the form of cyclic strain modulates proliferative capacity and integrin expression of chondrocytes from osteoarthritic knee joints. Chondrocytes isolated from articular cartilage during total knee arthroplasty were propagated on flexible silicone membranes. The cells were subjected to cyclic strain for 24 h using a computer-controlled vacuum device, with replicate samples maintained under static conditions. Our results demonstrated increase in proliferative capacity of the cells subjected to cyclic strain compared with cells maintained under static conditions. The flexed cells also exhibited upregulation of the chondrocytic gene markers type II collagen and aggrecan. In addition, cyclic strain resulted in increased expression of the α2 and α5 integrin subunits, as well as an increased expression of vimentin. There was also intracellular reconfiguration of the enzyme protein kinase C. Our findings suggest that these molecules may play a role in the signal transduction pathway, eliciting cellular response to mechanical stimulation.

Original languageEnglish (US)
Pages (from-to)138-142
Number of pages5
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume40
Issue number5-6
DOIs
StatePublished - May 1 2004

Keywords

  • Collagen type II
  • Extracellular matrix
  • Mechanical stimulation
  • Proliferation
  • Protein kinase C
  • Vimentin

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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