Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery

Amir Nasrolahi Shirazi, Karissa L. Paquin, Niall G. Howlett, Dindyal Mandal, Keykavous Parang

Research output: Contribution to journalArticle

Abstract

Previously, we have reported the synthesis of a homochiral L-cyclic peptide [WR]5 and its use for delivery of anti-HIV drugs and biomolecules. A physical mixture of HAuCl4 and the peptide generated peptide-capped gold nanoparticles. Here, [WR]5 and [WR]5-AuNPs were tested for their efficiency to deliver a small interfering RNA molecule (siRNA) in human cervix adenocarcinoma (HeLa) cells. Flow cytometry investigation revealed that the intracellular uptake of a fluorescence-labeled non-targeting siRNA (200 nM) was enhanced in the presence of [WR]5 and [WR]5-AuNPs by 2- and 3.8-fold when compared with that of siRNA alone after 24 h incubation. Comparative toxicity results showed that [WR]5 and [WR]5-AuNPs were less toxic in cells compared to other available carrier systems, such as Lipofectamine.

Original languageEnglish (US)
Pages (from-to)13319-13331
Number of pages13
JournalMolecules
Volume19
Issue number9
DOIs
StatePublished - Aug 28 2014
Externally publishedYes

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Keywords

  • Cyclic peptides
  • Gold nanoparticles
  • SiRNA delivery systems (DDS)
  • Small interfering RNA (siRNA) delivery

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

Cite this

Shirazi, A. N., Paquin, K. L., Howlett, N. G., Mandal, D., & Parang, K. (2014). Cyclic peptide-capped gold nanoparticles for enhanced siRNA delivery. Molecules, 19(9), 13319-13331. https://doi.org/10.3390/molecules190913319