Cyclic-nucleotide-gated cation current and Ca2+-activated Cl current elicited by odorant in vertebrate olfactory receptor neurons

Rong Chang Li, Yair Ben-Chaim, King Wai Yau, Chih Chun Lin

Research output: Contribution to journalArticle

Abstract

Olfactory transduction in vertebrate olfactory receptor neurons (ORNs) involves primarily a cAMP-signaling cascade that leads to the opening of cyclic-nucleotide-gated (CNG), nonselective cation channels. The consequent Ca2+ influx triggers adaptation but also signal amplification, the latter by opening a Ca2+-activated Cl channel (ANO2) to elicit, unusually, an inward Cl current. Hence the olfactory response has inward CNG and Cl components that are in rapid succession and not easily separable. We report here success in quantitatively separating these two currents with respect to amplitude and time course over a broad range of odorant strengths. Importantly, we found that the Cl current is the predominant component throughout the olfactory dose-response relation, down to the threshold of signaling to the brain. This observation is very surprising given a recent report by others that the olfactory-signal amplification effected by the Ca2+-activated Cl current does not influence the behavioral olfactory threshold in mice.

Original languageEnglish (US)
Pages (from-to)11078-11087
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number40
DOIs
StatePublished - Oct 4 2016

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Keywords

  • Calcium-activated chloride channel
  • Cyclic-nucleotide-gated channel
  • Olfactory receptor neurons
  • Olfactory transduction
  • Signal amplification

ASJC Scopus subject areas

  • General

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