Cyclic GMP-sensitive conductance in outer segment membrane of catfish cones

Lawrence Haynes, King Wai Yau

Research output: Contribution to journalArticlepeer-review

Abstract

A cyclic GMP-sensitive conductance has recently been observed with patch-clamp recording in excised inside-out patches of plasma membrane from frog and toad rod outer segments1,2. This conductance has properties suggesting that it is probably the light-sensitive conductance involved in visual transduction1,2. We now report a similar conductance in the outer segment membrane of catfish cones. Cyclic GMP showed positive cooperatively in opening this conductance, with a Hill coefficient of 1.6-3.0 and a half-saturating cGMP concentration of 35-70 μM. Cyclic AMP at 1 mM, or changing Ca concentration (in the presence of Mg), had little effect on the conductance. In physiological solutions the cGMP-induced current had a reversal potential near +10 mV; the current amplitude increased roughly exponentially with membrane potential in both depolarizing and hyperpolarizing directions. Our results suggest that cGMP is also the internal transmitter for phototransduction in cones.

Original languageEnglish (US)
Pages (from-to)61-64
Number of pages4
JournalNature
Volume317
Issue number6032
DOIs
StatePublished - Dec 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • General

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