Cyclic caged morpholinos: Conformationally gated probes of embryonic gene function

Sayumi Yamazoe, Ilya A. Shestopalov, Elayne Provost, Steven D. Leach, James K. Chen

Research output: Contribution to journalArticlepeer-review

Abstract

Feeling a bit cagey: Morpholino-based antisense reagents have been caged through oligonucleotide cyclization (see scheme), enabling photocontrol of gene expression in zebrafish embryos and larvae. Using these reagents, the timing of exocrine cell fate commitment in the developing pancreas has been examined.

Original languageEnglish (US)
Pages (from-to)6908-6911
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number28
DOIs
StatePublished - Jul 9 2012

Keywords

  • antisense oligonucleotides
  • developmental biology
  • gene expression
  • oligonucleotides
  • zebrafish

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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