Cyclic AMP raises cytosolic Ca2+ and promotes Ca2+ influx in a clonal pancreatic β-cell line (HIT T-15)

Marc Prentki, Major C. Glennon, Jean François Geschwind, Franz M. Matschinsky, Barbara E. Corkey

Research output: Contribution to journalArticlepeer-review


The effect on cytosolic Ca2+ concentration ([Ca2+]i) of cAMP analogues and the adenylate cyclase-stimulating agents forskolin, isoproterenol and glucagon has been examined in an insulin-secreting β-cell line (HIT T-15) using fura 2. All these manipulations of the cAMP messenger system promoted a rise in [Ca2+]i which was blocked by the Ca2+ channel antagonists verapamil and nifedipine or by removal of extracellular Ca2+. The action of the adenylate cyclase activator forskolin was glucose-dependent. The results suggest that cAMP elevates [Ca2+]i in HIT cells by promoting Ca2+ entry through voltage-sensitive Ca2+ channels, not through mobilization of stored Ca2+. Activation of Ca2+ influx may be an important component of the mechanisms by which cAMP potentiates fuel-induced insulin release.

Original languageEnglish (US)
Pages (from-to)103-107
Number of pages5
JournalFEBS Letters
Issue number1
StatePublished - Aug 10 1987


  • Ca channel blocker
  • D-Glucose
  • Forskolin
  • Insulin release
  • cyclic AMP
  • cytosolic free Ca

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


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