TY - JOUR
T1 - Cyclic AMP metabolism in asthma
T2 - Studies with leukocytes and lymphocytes
AU - Gillespie, Elizabeth
AU - Valentine, Martin D.
AU - Lichtenstein, Lawrence M.
N1 - Funding Information:
The subjects used in the present study were patients or employees at The Good Samaritan Hospital, Baltimore, Maryland. Two groups of asthmatic individuals were studied. The first group, used for the lymphocyte experiments, all had active asthma as defined by history. All had had at least one major exacerbation of asthma in the previous three months, and most required daily medication to avoid the emergence of bronchospasm. This group was subdivided From The Johns Hopkins University School of Medicine at The Good Samaritan Hospital. Supported by Grants Nos. HL 14153, AI 10304, AI 108270, and AI 42373, National Institutes of Health. Publication No. 93, the O’Neill Research Laboratories. Received for publication June 28, 1973. Reprint requests to: Dr. Elizabeth Gillespie, The Good Samaritan Hospital, 5601 Loch Raven Blvd., Baltimore, Md. 21239. *Research Career Development Awardee, National Institute of Allergy and Infectious Diseases.
PY - 1974/1
Y1 - 1974/1
N2 - The effect of isoproterenol on cyclic AMP (cAMP) levels in lymphocytes and leukocytes from asthmatic and normal individuals has been studied. Lymphocyte cAMP levels rose in response to 10-8 to 10-2 M isoproterenol; the dose-response curve was biphasic with a maximum response at concentrations of 10-6 to 10-4 M. At all drug concentrations the response of cells from asthmatic individuals was less than the response of cells from normal control subjects. The difference between the two groups was not statistically significant, however. Similarly, basal cAMP levels were lower in the cells of asthmatic as compared with normal individuals, but again the difference was not significant. When these two parameters were combined and the results expressed as absolute cAMP levels after isoproterenol treatment, a significant difference was observed. Both the unstimulated. cAMP levels and the response to isoproterenol of leukocytes from normal and asthmatic subjects were influenced by the incubation medium. Use of a medium buffered with tris as compared with bicarbonate-phosphate resulted in lower basal cAMP levels and increased responsiveness to isoproterenol. Treatment of normal leukocytes with serum from asthmatic individuals did not alter their response to isoproterenol.
AB - The effect of isoproterenol on cyclic AMP (cAMP) levels in lymphocytes and leukocytes from asthmatic and normal individuals has been studied. Lymphocyte cAMP levels rose in response to 10-8 to 10-2 M isoproterenol; the dose-response curve was biphasic with a maximum response at concentrations of 10-6 to 10-4 M. At all drug concentrations the response of cells from asthmatic individuals was less than the response of cells from normal control subjects. The difference between the two groups was not statistically significant, however. Similarly, basal cAMP levels were lower in the cells of asthmatic as compared with normal individuals, but again the difference was not significant. When these two parameters were combined and the results expressed as absolute cAMP levels after isoproterenol treatment, a significant difference was observed. Both the unstimulated. cAMP levels and the response to isoproterenol of leukocytes from normal and asthmatic subjects were influenced by the incubation medium. Use of a medium buffered with tris as compared with bicarbonate-phosphate resulted in lower basal cAMP levels and increased responsiveness to isoproterenol. Treatment of normal leukocytes with serum from asthmatic individuals did not alter their response to isoproterenol.
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U2 - 10.1016/0091-6749(74)90096-7
DO - 10.1016/0091-6749(74)90096-7
M3 - Article
C2 - 4357825
AN - SCOPUS:0015979602
SN - 0091-6749
VL - 53
SP - 27
EP - 33
JO - Journal of Allergy and Clinical Immunology
JF - Journal of Allergy and Clinical Immunology
IS - 1
ER -