Cyclic AMP mediates the negative regulation of Y-organ ecdysteroid production

Mark P. Mattson; Eugene Spaziani

doi:10.1016/0303-7207(85)90106-6

Cyclic AMP mediates the negative regulation of Y-organ ecdysteroid production

Mark P. Mattson, Eugene Spaziani

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The putative neuropeptide, molt-inhibiting hormone (MIH), regulates crustacean growth by periodically suppressing the secretion of ecdysteriod molting hormone from peripheral glands (Y-organs). A mediating role for cyclic AMP (cAMP) in MIH action was evaluated with isolated Y-organs of the crab, Cancer antennarius. MIH activity in eyestalk extracts inhibited ecdysteroid secretion but increased cAMP levels dose-dependently in 24-h incubations. The cAMP rise preceded the onset of ecdysteroid suppression. Dibutyryl cAMP, activators of adenylate cyclase (forskolin, choleragen), and an inhibitor of phosphodiesterase (IBMX), but not AMP or cGMP, mimicked the inhibitory action of MIH.

Original language	English (US)
Pages (from-to)	185-189
Number of pages	5
Journal	Molecular and Cellular Endocrinology
Volume	42
Issue number	2
DOIs	https://doi.org/10.1016/0303-7207(85)90106-6
State	Published - 1985
Externally published	Yes

Keywords

Cancer antennarius
choleragen
crustaceans
forskolin
molt-inhibiting hormone
steroidogenesis

ASJC Scopus subject areas

Endocrinology
Endocrinology, Diabetes and Metabolism

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10.1016/0303-7207(85)90106-6

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title = "Cyclic AMP mediates the negative regulation of Y-organ ecdysteroid production",

abstract = "The putative neuropeptide, molt-inhibiting hormone (MIH), regulates crustacean growth by periodically suppressing the secretion of ecdysteriod molting hormone from peripheral glands (Y-organs). A mediating role for cyclic AMP (cAMP) in MIH action was evaluated with isolated Y-organs of the crab, Cancer antennarius. MIH activity in eyestalk extracts inhibited ecdysteroid secretion but increased cAMP levels dose-dependently in 24-h incubations. The cAMP rise preceded the onset of ecdysteroid suppression. Dibutyryl cAMP, activators of adenylate cyclase (forskolin, choleragen), and an inhibitor of phosphodiesterase (IBMX), but not AMP or cGMP, mimicked the inhibitory action of MIH.",

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T1 - Cyclic AMP mediates the negative regulation of Y-organ ecdysteroid production

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AU - Spaziani, Eugene

PY - 1985

Y1 - 1985

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AB - The putative neuropeptide, molt-inhibiting hormone (MIH), regulates crustacean growth by periodically suppressing the secretion of ecdysteriod molting hormone from peripheral glands (Y-organs). A mediating role for cyclic AMP (cAMP) in MIH action was evaluated with isolated Y-organs of the crab, Cancer antennarius. MIH activity in eyestalk extracts inhibited ecdysteroid secretion but increased cAMP levels dose-dependently in 24-h incubations. The cAMP rise preceded the onset of ecdysteroid suppression. Dibutyryl cAMP, activators of adenylate cyclase (forskolin, choleragen), and an inhibitor of phosphodiesterase (IBMX), but not AMP or cGMP, mimicked the inhibitory action of MIH.

KW - Cancer antennarius

KW - choleragen

KW - crustaceans

KW - forskolin

KW - molt-inhibiting hormone

KW - steroidogenesis

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Cyclic AMP mediates the negative regulation of Y-organ ecdysteroid production

Abstract

Keywords

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